PMID: 8598672Feb 1, 1996Paper

The effect of trinitrobenzene sulfonic acid (TNB) on colonocyte arachidonic acid metabolism

The Journal of Surgical Research
M D StrattonW E Longo

Abstract

In previous studies we found that luminal perfusion of the isolated left colon of the rabbit with the hapten, trinitrobenzene, resulted in the production of an acute inflammatory process associated with alterations in eicosanoid metabolism. As the colitis was attenuated by cyclooxygenase inhibitors it is possible that the inflammation was mediated by arachidonic acid metabolites. In the present study it was intended to evaluate the effect of trinitrobenzene on eicosanoid metabolism in transformed human colonic cells by exposing Caco-2++ cells to various doses of trinitrobenzene. Cell injury was evaluated by measuring lactate dehydrogenase levels and cyclooxygenase and lipoxygenase activity was evaluated by measuring prostanoid and leukotriene production. In separate experiments resting and trinitrobenzene stimulated cells were treated with indomethacin and dexamethasone. Trinitrobenzene produced increased prostaglandin E2 and 6-keto prostaglandin F1alpha++ and increased lactate dehydrogenase levels. Leukotriene B4 was significantly increased compared to control values at the highest TNB concentration administered. Indomethacin inhibited the lactate dehydrogenase and prostanoid changes, suggesting that the inflammatory changes p...Continue Reading

Citations

Aug 2, 2001·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·M UsamiA Miki
Nov 26, 1999·Journal of Gastroenterology and Hepatology·B W ChenX G Liu

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