PMID: 3213639Jan 1, 1988Paper

The effect of type beta transforming growth factor on proliferation of clonogenic cells from human gliomas

Acta neurochirurgica. Supplementum
E HelsethR Vik

Abstract

Type beta transforming growth factor (B-TGF) is a potent regulator of cell growth and differentiation. Growth of many human tumour cell lines are inhibited by B-TGF. The effect of B-TGF on proliferation of clonogenic cells from 12 human glioma biopsies was registrated in a thymidine incorporation assay. B-TGF appeared to be a potent growth inhibitor for some gliomas, while it had no effect on others. Maximum inhibition was about 60%. Though not significant, glioblastomas appeared to be less sensitive to inhibition by B-TGF than astrocytomas and oligodendrogliomas. Very little is known about the growth inhibitory action of B-TGF. The negative autocrine growth theory for cancer cells postulates that a reduced production, or production of a defective growth inhibitor normally found in the cell, may account for the autonomous nature of some cancer cells. In view of this theory, we searched for B-TGF in protein extracts from a glioblastoma cell line, T-MGl, whose growth was inhibited by B-TGF. Protein extract from T-MGl cells was analysed for B-TGF activity using a soft agar colony formation assay with normal rat kidney fibroblasts. B-TGF was not found in the extract. Since, according to the literature B-TGF has been found in all ot...Continue Reading

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