The effect of ulinastatin on the small intestine injury and mast cell degranulation in a rat model of sepsis induced by CLP

Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie
Yi-Jing ZhangCheng-Yong Qin

Abstract

Sepsis could be initiated by the gastrointestinal tract injury and subsequent bacterial translocation. In the present experiment, we aimed to investigate effect of ulinastatin (UTI) on the small intestinal injury and bacterial translocation in septic rats and role of mast cells degranulation in its action. Fifty-four male Wistar rats were randomly divided into three groups: sham laparatomy, cecal ligation and puncture (CLP), and CLP plus UTI. CLP was used to develop septic rat model and UTI was administered to rats intraperitoneally (50,000 U/kg) 30 min prior to CLP operation. After CLP or sham operation, variable parameters were investigated in three subsets of animals. One subset was used for measurements of nitrite and nitrate (NO(x)) concentration in plasma at 1, 6, 12, 18, and 24h and levels of NO(x) and iNOS mRNA in the small intestine, RMCP-II released into the small intestinal lumen, bacterial translocation and morphologic changes at 24h. The other subsets were used for the small intestinal motility and microvascular in vivo at 24h. Bacterial translocation, barrier injury, impaired motility and blood flow, mast cells degranulation of the small intestine in the CLP group were found more severe than that in the sham group...Continue Reading

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Citations

Nov 7, 2017·Oxidative Medicine and Cellular Longevity·Pia RademannMarcin F Osuchowski

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