PMID: 326709May 1, 1977Paper

The effect of U.V.-irradiation on lambda DNA transcription

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
S S Ranade

Abstract

The effect of U.V.-irradiation of template DNA has been studied in vitro in the E. coli RNA polymerase system with native and U.V.-treated lambda DNA. Lambda DNA is more susceptible to U.V. than is calf-thymus DNA, yet a residual activity is observed at a U.V. dose of 0-5+10(4) erg/mm2. From the kinetic analysis of the reaction and the incorporation of lambda 32P-labelled nucleoside triphosphates, it seems reasonable to conclude that U.V.-irradiation probably does not affect the DNA initiation sites, recognizable by RNA polymerase. The transcription products made with U.V.-irradiated lambda DNA are assymmetrical, and hybridized to the right half (R) and the left half (L) of lambda DNA with the ratio of R/L = 4/1, and they show a lower hybridizability than the transcripts with native lambda DNA. The initiation sites recognizable by RNA polymerase seem to be the same on both native and U.V.-irradiated lambda DNA though the transcription of U.V.-treated lambda DNA appears to terminate with rather short RNA chains.

References

Jan 25, 1975·Journal of Molecular Biology·P B Hackett, W Sauerbier
Jan 17, 1973·Nature: New Biology·C F Brunk
Jan 1, 1966·Cold Spring Harbor Symposia on Quantitative Biology·U MaitraJ Hurwitz
Jan 1, 1966·Cold Spring Harbor Symposia on Quantitative Biology·S Naono, F Gros
Oct 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·A G SoA Tissières
Jul 1, 1965·Journal of Molecular Biology·D Gillespie, S Spiegelman
Jan 6, 1970·Biochemical and Biophysical Research Communications·H Chessin, W C Summers
Jul 15, 1960·Biochimica Et Biophysica Acta·R BEUKERS, W BERENDS

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