The effect of vinblastine on the incorporation of [3H]-glycine into proteins of the periodontal ligament of impeded and unimpeded mouse incisors

Archives of Oral Biology
M L Polacow, J Merzel

Abstract

The effect of vinblastine on the protein metabolism of the periodontal ligament of impeded and unimpeded mouse incisors was studied by [3H]-glycine labelling and radioautography. The silver-grain concentration was determined in areas adjacent to the tooth, areas adjacent to bone and, as an internal control, in the dentine matrix. From 1 to 12 h there was no difference between treated and control animals; thus the drug did not alter protein biosynthesis. Later, the silver-grain concentration was significantly higher in areas adjacent to both bone and tooth in vinblastine-treated animals, suggesting a longer half-life of the labelled proteins. No significant differences between normal or unimpeded erupting incisors of both groups were detected. Dentine matrix showed a possibly higher re-utilization of the labelled amino acid in vinblastine-treated animals. The amount of labelled protein removed by collagenase was similar in both groups, while the concentration of grains due to collagenase-resistant proteins was significantly higher in treated animals, particularly at 96 h after the injection of labelled glycine. The relation between the increased amount of non-collagenous proteins in the periodontal ligament and the decrease in t...Continue Reading

References

Jan 1, 1978·Archives of Oral Biology·W A Orlowski
Nov 1, 1977·The Anatomical Record·W Beertsen, V Everts
Jan 1, 1986·Archives of Oral Biology·A A TaverneG J Tonino
Sep 1, 1967·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H Warshawsky, G Moore

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