The effect of vitamin C on N-acetyltransferase activity in Klebsiella pneumoniae

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
S E HsiehJ G Chung

Abstract

This study was designed to assess the effect of vitamin C on arylamine N-acetyltransferase (NAT) activity in Klebsiella pneumoniae by using HPLC to measure the acetylation of 2-aminofluorene (2-AF) with and without vitamin C. Two assay systems were performed, one with intact bacterial cell suspensions, the other with S-9 fractions (9000g supernatant). It was found that vitamin C promoted NAT activity in K. pneumoniae in a dose-dependent manner in both systems. 4 and 8 mM vitamin C were selected for further studies in S-9 fractions and intact cell systems, respectively. Through a 4-hr time course study, vitamin C promoted the N-acetylation of 2-AF in both assay systems, but, the longer the reaction time lasted, the lower the promotion rate. In the kinetic studies, vitamin C increased the value of Km from 0.42+/-0.03 mM to 2.43+/-0.87 mM in S-9 fraction assays and from 0.54+/-0.03 mM to 0.85+/-0.18 mM in intact cell assays. Vitamin C also increased the apparent Vmax values from 3.5 +/-0.08 to 39.66+/-9.81 nmol/min/mg protein in S-9 fraction assays, and from 1.28+/-0.06 to 4.88+/-0.87 nmol/min/10(10) CFU in intact cell assays, for acetylation of 2-AF. In the presence of vitamin C, the NAT activity was increased from 0.58+/-0.06 to...Continue Reading

References

Jan 1, 1986·Journal of Toxicology and Environmental Health·B W ManningT W Federle
Oct 1, 1984·Cell Biology and Toxicology·J A Holme, E J Søderlund
Dec 1, 1993·International Journal of Epidemiology·J M RamónJ Oromí
Dec 1, 1993·Oral Surgery, Oral Medicine, and Oral Pathology·J SchwartzD Trickler

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Citations

Sep 9, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J G Chung
Nov 1, 2005·Drug Metabolism Reviews·Sotiria Boukouvala, Giannoulis Fakis
Dec 5, 1998·Drug and Chemical Toxicology·J G ChungJ M Lai

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