The Effect of Volatile Oil from Vernonia anthelmintica Seeds on Melanin Synthesis in B16 Cells and Its Chemical Analysis by GC-QTOF-MS

Evidence-based Complementary and Alternative Medicine : ECAM
Abulikemu AobuliH A Aisa

Abstract

Vernonia anthelmintica Willd. seeds have been used in folk medicine for the treatment of leukoderma in Xinjiang, China, for more than 300 years. The promoting activities of its volatile oil (AVO) in melanogenesis and its chemical composition were investigated in this paper. The bioactivities of AVO were examined by melanin synthesis and tyrosinase activity assay in B16 cells. Using GC-QTOF-MS technology, each compound of AVO contains a single separated peak in GC and the retention indices of every GC peak were calculated by the retention times of C7~C30 n-alkanes that were injected at the same chromatographic conditions. Then each individual peak was identified by comparing its mass spectrum with the MS library (NIST 14). As a result, AVO increased the melanin content and tyrosinase activity in a dose-dependent manner at concentrations of 10-30μg·mL-1. The 64 compounds were identified in AVO which occupied 95.15% of total peak area in GC. They mainly contained caryophyllene (23.73%), sabinene (18.15%), α-thujene (6.57%), thymol (5.29%), 4-epi-α-acoradiene (4.98%), limonene (4.92%), anethole (3.44%), etc. According to the results the AVO can promote melanogenesis and upregulate tyrosinase activity in B16 cells.

References

Nov 10, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Shaik Sameena FatimaChippada Appa Rao
Jun 15, 2010·Current Drug Metabolism·Liliana GuerraDesanka Raskovic
Nov 19, 2013·Current Opinion in Immunology·Jillian M RichmondJohn E Harris
Jul 17, 2014·The Journal of Investigative Dermatology·Lei QiuVijayasaradhi Setaluri
Jan 19, 2015·Lancet·Khaled EzzedineNanja van Geel
Apr 29, 2015·Fitoterapia·Ablajan TurakHaji Akber Aisa
Jul 2, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Saad S DahhamAmin M S A Majid
Sep 22, 2015·Journal of Dermatological Science·Heng XieTianwen Gao
Apr 8, 2017·Journal of Dermatological Science·Yuqi YangZhe Jian
Apr 21, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Mallappa Kumara SwamyUma Rani Sinniah
Sep 14, 2017·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Wen-Wan ChaoSu-Tze Chou
Sep 16, 2017·Acta Biochimica Et Biophysica Sinica·Nuramina MamatAisa Haji Akber
Jan 3, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Piao ZhengJiekun Luo

❮ Previous
Next ❯

Citations

Aug 13, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shilpi Goenka, Sanford R Simon
May 1, 2021·International Journal of Molecular Sciences·Yifan LaiHui Zhong

❮ Previous
Next ❯

Methods Mentioned

BETA
Protein Assay

Software Mentioned

Mass Hunter Qualitative Analysis

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.