The Effect of Weight Loss on the Muscle Proteome in the Damara, Dorper and Australian Merino Ovine Breeds

PloS One
André M AlmeidaL A Cardoso

Abstract

Seasonal Weight Loss (SWL) is an important constraint, limiting animal production in the Tropics and the Mediterranean. As a result, the study of physiological and biochemical mechanisms by which domestic animal breeds respond to SWL is important to those interested in animal breeding and the improvement thereof. To that end, the study of the proteome has been instrumental in gathering important information on physiological mechanisms, including those underlying SWL. In spite of that, little information is available concerning physiological mechanisms of SWL in production animals. The objective of this study was to determine differential protein expression in the muscle of three different breeds of sheep, the Australian Merino, the Dorper and the Damara, each showing different levels of tolerance to weight loss (low, medium and high, respectively). Per breed, two experimental groups were established, one labeled "Growth" and the other labeled "Restricted." After forty-two days of dietary treatment, all animals were euthanized. Muscle samples were then taken. Total protein was extracted from the muscle, then quantified and two-dimensional gel electrophoresis were conducted using 24 cm pH 3-10 immobiline dry strips and colloidal ...Continue Reading

References

Jan 4, 2005·Proteomics·Julien BouleyBrigitte Picard
Nov 10, 2006·Journal of Animal Science·M HamelinE Laville
Dec 15, 2006·Tropical Animal Health and Production·A M AlmeidaL A Cardoso
Aug 22, 2008·Tropical Animal Health and Production·André Martinho de Almeida, Luís Alfaro Cardoso
Aug 22, 2008·Tropical Animal Health and Production·André Martinho de Almeida, Luís Alfaro Cardoso
May 8, 2009·Journal of Animal Science·M ShibataK Chikuni
Feb 15, 2011·Biochimica Et Biophysica Acta·Juliette MartinJean-François Dufour
Apr 22, 2011·Tropical Animal Health and Production·André Martinho Almeida
Sep 1, 2003·Meat Science·P A TshabalalaH L de Kock
May 1, 2012·Journal of Proteomics·Gianluca ParediAndrea Mozzarelli
May 1, 2012·Journal of Proteomics·André Martinho de Almeida, Emøke Bendixen
May 29, 2012·Journal of Proteomics·Peter David EckersallIngrid Miller
Oct 4, 2012·Animal : an International Journal of Animal Bioscience·S van HartenL A Cardoso
Jan 25, 2013·Tropical Animal Health and Production·André M AlmeidaJohan C Greeff
Feb 14, 2013·Journal of Proteomics·Gianluca ParediAndré Martinho de Almeida
May 15, 2013·Metabolism: Clinical and Experimental·Maria H HolmströmJuleen R Zierath
Oct 1, 2013·Journal of Proteomics·Ana M FerreiraAndré M Almeida
Dec 12, 2013·Cell and Tissue Research·Richard FairlessRicarda Diem
Feb 22, 2014·Current Protein & Peptide Science·Ingrid MillerAndre M de Almeida
Apr 9, 2014·BMC Veterinary Research·Lorenzo Enrique Hernández-CastellanoAnastasio Argüello
Apr 9, 2014·Journal of Proteomics·André Martinho AlmeidaStefan Clerens
Nov 2, 2014·Animal : an International Journal of Animal Bioscience·A M AlmeidaR Turk

❮ Previous
Next ❯

Citations

Jul 10, 2018·Animal : an International Journal of Animal Bioscience·J PlowmanA M de Almeida
Mar 14, 2020·Journal of Animal Physiology and Animal Nutrition·David Miguel RibeiroAndré Martinho de Almeida
Jun 30, 2019·Journal of Animal Physiology and Animal Nutrition·David M RibeiroAndré M Almeida
Feb 6, 2020·Scientific Reports·Xinyue WangLi Zhang
May 1, 2021·Animals : an Open Access Journal From MDPI·Mousa ZarrinLorenzo E Hernández-Castellano

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis

Software Mentioned

Progenesis SameSpots
STATISTICA
Expasy Mw / pI Tool
- demon
GPS Explorer
Imagemaster
4000 Series Explorer
Mowse
MASCOT

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.