PMID: 8939462Nov 1, 1996Paper

The effect of X-radiation on cerebellar granule cells grown in culture. Ganglioside GM1 neuroprotective activity

Neurochemistry International
Laura Ruth GuelmanM L Fiszman

Abstract

In this paper we describe the effects of X-radiation on the viability of cerebellar granule cells grown in culture. Cell cultures were exposed to X-rays 2 h after plating and then grown for 1-7 days. Two days after X-ray exposure with a dose-range of 0.1-2 Gy (acute effect), a significant decrease in neuronal number was observed. The magnitude of the lethal effect was directly correlated to the dose of X-ray applied. When the interval between plating and irradiation was increased, the acute lethal effect of X-rays decreased. 3H-thymidine incorporation was maximal during the first 24 h in vitro and decreased to nearly blank levels, after 72 h. In some experiments, cells present in each culture dish were counted at day 2 and at day 7. We observed that the number of cells present in sham-irradiated cultures decreased from day 2 to day 7, reflecting cell death after several days in vitro. The cell loss observed in X-irradiated cultures was significantly greater as compared with sham-irradiated cultures, confirming the deleterious effect of X-ray on cell survival. This effect was completely prevented by GM1 (6.5, 10 and 30 microM) added 48 h after X-ray exposure, but not 1 h after plating. We conclude that X-rays induce two differen...Continue Reading

References

Aug 1, 1990·Molecular and Chemical Neuropathology·A M DopicoL M Zieher
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·F VaccarinoE Costa
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M FavaronE Costa
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A C CuelloD Maysinger
Jan 1, 1986·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·G ToffanoK G Fuxe
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·V GalloG Levi
Jan 1, 1993·Journal of Neuroscience Research·E A NealeT G Smith
May 1, 1993·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·G FerrariL A Greene

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Citations

Apr 29, 2004·Neurotoxicology and Teratology·Laura Ruth GuelmanLuis María Zieher
May 29, 1998·Brain Research. Developmental Brain Research·L N Borodinsky, M L Fiszman
Dec 24, 2011·PloS One·Anandh Dhanushkodi, Michael P McDonald
Oct 25, 2008·Ecotoxicology and Environmental Safety·Lucila Guadalupe CaceresLaura Ruth Guelman
Dec 18, 2010·Neurochemistry International·Maria A Zorrilla ZubileteAna M Genaro
Aug 23, 2001·Medical Hypotheses·C ThomasB Fertil
Feb 26, 2005·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Laura Ruth GuelmanLuis María Zieher
Jun 28, 2005·Journal of Neuropathology and Experimental Neurology·Lisa NowoslawskiKevin A Roth

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