The effect on MHC class II expression and apoptosis in placenta by IFNgamma administration

Contraception
Zhe LiuJing-pian Peng

Abstract

To investigate the possible mechanisms by which interferon gamma (IFNgamma) affects pregnancy, the expression of class II major histocompatibility complex (MHC) antigens and cleavage of DNA that is a hallmark of apoptosis in the placenta were examined by molecular biochemical techniques, and progesterone levels were examined by radioimmunoassay. The semi-quantitative analysis with reverse transcription-polymerase chain reaction showed that the expression of MHC class II antigen in placenta increased when rabbits were treated with high doses of IFNgamma compared with the control. However, immunohistochemical study suggested that IFNgamma did not affect MHC class II expression in trophoblasts, but had a stimulatory effect on its expression in maternal decidua and placental lymphocytes. DNA fragmentation analysis and terminal deoxynucleotidyl mediated-deoxyuridine triphosphate nick end labeling (TUNEL) assay indicated that the cleavage of DNA was detected in the placenta in both normal and IFNgamma-treated pregnancy. Quantitative analysis of apoptotic cells revealed an increase in trophoblasts treated with IFNgamma compared to those in normal pregnancy. Moreover, progesterone, which plays an important role in pregnancy, was reduce...Continue Reading

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Citations

May 10, 2003·Fertility and Sterility·Ulrike von RangoHenning M Beier
Jul 27, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Quan-Hong SunYing Yang
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Nov 28, 2012·Journal of Reproductive Immunology·Katerina N BambangJustin C Konje
Jul 23, 2014·Asian-Australasian Journal of Animal Sciences·Li-Fang SiXiang-Chao Cheng
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Oct 31, 2017·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Hao ZhangHong-Juan Peng
Dec 15, 2020·American Journal of Reproductive Immunology : AJRI·Olivia K TravisDenise C Cornelius

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis