The effective temperature of Peptide ions dissociated by sustained off-resonance irradiation collisional activation in fourier transform mass spectrometry.

The Journal of Physical Chemistry. B
P D SchnierEvan R Williams

Abstract

A method for determining the internal energy of biomolecule ions activated by collisions is demonstrated. The dissociation kinetics of protonated leucine enkephalin and doubly protonated bradykinin were measured using sustained off-resonance irradiation (SORI) collisionally activated dissociation (CAD) in a Fourier transform mass spectrometer. Dissociation rate constants are obtained from these kinetic data. In combination with Arrhenius parameters measured with blackbody infrared radiative dissociation, the "effective" temperatures of these ions are obtained. Effects of excitation voltage and frequency and the ion cell pressure were investigated. With typical SORI-CAD experimental conditions, the effective temperatures of these peptide ions range between 200 and 400 degrees C. Higher temperatures can be easily obtained for ions that require more internal energy to dissociate. The effective temperatures of both protonated leucine enkephalin and doubly protonated bradykinin measured with the same experimental conditions are similar. Effective temperatures for protonated leucine enkephalin can also be obtained from the branching ratio of the b(4) and (M + H - H(2)O)(+) pathways. Values obtained from this method are in good agreem...Continue Reading

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Citations

Dec 29, 2011·Journal of the American Society for Mass Spectrometry·Samuel I MerenbloomEvan R Williams
Sep 6, 2012·Journal of the American Society for Mass Spectrometry·Tawnya G Flick, Evan R Williams
May 25, 2007·Journal of the American Society for Mass Spectrometry·Ryan D LeibEvan R Williams
Oct 26, 2007·Journal of the American Society for Mass Spectrometry·Csaba PeltzKároly Vékey
Mar 29, 2008·Journal of the American Society for Mass Spectrometry·Jeremy T O'BrienEvan R Williams
Dec 17, 2008·Journal of the American Society for Mass Spectrometry·James W Hager
Jan 29, 2010·Journal of the American Society for Mass Spectrometry·William A Donald, Evan R Williams
Jan 24, 2006·Journal of the American Chemical Society·S E Rodriguez-CruzE R Williams
Mar 24, 2006·Journal of the American Chemical Society·E F StrittmatterE R Williams
Feb 10, 2006·The Journal of Physical Chemistry. a·R A JockuschE R Williams
Sep 25, 2004·Mass Spectrometry Reviews·Julia Laskin, Jean H Futrell
Mar 28, 2009·Mass Spectrometry Reviews·Paul M Mayer, Clement Poon
Jul 30, 2010·Mass Spectrometry Reviews·Judit SztárayKároly Vékey
Apr 15, 2015·Journal of the American Society for Mass Spectrometry·Moritz Schennach, Kathrin Breuker
Nov 10, 2012·The Journal of Physical Chemistry. B·Jongcheol SeoSeung Koo Shin
Jul 3, 2003·Mass Spectrometry Reviews·Julia Laskin, Jean H Futrell
Nov 1, 2017·Journal of the American Society for Mass Spectrometry·Katrina BrendleManfred M Kappes
Aug 17, 2001·Journal of the American Society for Mass Spectrometry·G van der RestH E Audier
Jun 9, 2020·Journal of the American Society for Mass Spectrometry·Cameron N NaylorBrian H Clowers
Mar 5, 2020·Journal of the American Society for Mass Spectrometry·Micah T DonorJames S Prell
Mar 31, 2007·Journal of the American Chemical Society·Ryan D LeibEvan R Williams
Apr 8, 2006·The Journal of Physical Chemistry. B·Christopher F RodriquezK W Michael Siu
Feb 27, 2003·Journal of the American Chemical Society·John C Jurchen, Evan R Williams
Jun 17, 2004·Journal of the American Chemical Society·Joshua K HoernerIgor A Kaltashov
Sep 27, 2007·The Journal of Physical Chemistry. a·Isabelle Compagnon, Philippe Dugourd

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