The effects and mechanisms of a novel 2-aminosteroid on murine WEHI-3B leukemia cells in vitro and in vivo

Leukemia Research
Q He, X Na

Abstract

To evaluate the effects of a novel 2-aminosteroid, 2-(4'-methyl-1'-piperazinyl)-3alpha-hydroxyl-5alpha-androstane-17-one (KH), on in vitro murine WEHI-3B leukemia cells, semisolid colony culture, MTT assay, morphological examination, NBT reduction, NSAE test and ACP assay were used to determine proliferation and differentiation. It was found that the growth of leukemia cells in colony and liquid cultures was inhibited by KH (10(-8)-10(-4) mol/l) after treatment for 7 days. The percentages of NBT and NSAE positive cells were 71.17 and 79.25%, respectively, after treatment with KH (10(-8)-10(-6) mol/l) for 5 days. The morphology of treated leukemia cells was identified to be macrophage-like and these cells acquired significant ACP activities. It was indicated that the ACP enzyme activities were increased as high as two and three times of the control, respectively, after treatment with 10(-8) or 10(-5) mol/l KH for 6 days. It was also indicated by DNA fragmentation in gel electrophoresis that WEHI-3B cells were induced toward apoptosis by KH (10(-8)-10(-4) mol/l) when checked at day 5. The c-myc mRNA expressions in WEHI-3B cells were decreased by 58.7% after treatment with KH (10(-8) mol/l) for 5 days. Therefore, it is first repor...Continue Reading

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Citations

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May 19, 2009·Phytotherapy Research : PTR·Chun-Shu YuJing-Gung Chung
Sep 18, 2010·Leukemia & Lymphoma·Tung-Yuan LaiJing-Gung Chung
Nov 8, 2014·Journal of Agricultural and Food Chemistry·Wen-Chen ChuangFung-Jou Lu
Mar 6, 2007·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·J LiuW X Hu
Oct 24, 2009·Human & Experimental Toxicology·Fu-Shun YuJing-Gung Chung
Mar 4, 2010·Human & Experimental Toxicology·Jai-Sing YangJing-Gung Chung
May 7, 2016·Integrative Cancer Therapies·Hsin-Ling YangYou-Cheng Hseu
Feb 20, 2009·Chemical Communications : Chem Comm·Xianbing KeXianming Hu
Nov 2, 2002·British Journal of Pharmacology·Sanyang GaoHirohei Yamamura
Mar 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kazumi EbataShigeru Saito

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis