The effects of 3,4-diaminopyridine on acetylcholine release at the frog neuromuscular junction

European Journal of Pharmacology
N N Durant, I G Marshall

Abstract

The effects of 3,4-diaminopyridine (3,4-DAP) were studied on spontaneous and evoked transmitter release in the frog sartorius muscle preparation. At a concentration of 1 mM, 3,4-DAP caused the appearance of spontaneous giant potentials, this effect of 3,4-DAP also occurred in absence of extracellular calcium. At lower concentrations in magnesium Ringer 3,4-DAP (1-10 microM) increased e.p.p. quantal content without causing the appearance of spontaneous giant potentials, 3,4-DAP prolonged muscle action potential (1 mM) and it is suggested that prolongation of the nerve terminal action potential by 3,4-DAP contributes largely to the increased evoked acetylcholine release. The ability of 3,4-DAP to cause the appearance of giant spontaneous potentials is considered to be independent of the drug's known ability to inhibit potassium conductance in excitable membranes, and may be due to the release of performed multiquantal packets of acetylcholine as suggested for vinblastine. It is concluded that 3,4-DAP is a useful tool in the study of both evoked and spontaneous acetylcholine release.

References

Jan 1, 1977·Comparative Biochemistry and Physiology. C: Comparative Pharmacology·A L Harvey, I G Marshall
Apr 1, 1978·The Journal of Pharmacy and Pharmacology·Y N SinghA L Harvey
Jul 15, 1979·European Journal of Pharmacology·J MolgóP Lechat
Aug 31, 1979·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·B Katz, R Miledi
Jul 1, 1975·British Journal of Pharmacology·I G Marshall, R L Parsons
Mar 14, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·W C BowmanI G Marshall
Aug 15, 1977·European Journal of Pharmacology·A L Harvey, I G Marshall
Jun 1, 1978·Biophysical Journal·G E Kirsch, T Narahashi
Nov 1, 1976·The Journal of General Physiology·J Z YehT Narahashi
Jan 1, 1976·Biophysical Journal·J Z YehT Narahashi

❮ Previous
Next ❯

Citations

Jun 1, 1992·Pflügers Archiv : European journal of physiology·T M ArgentieriJ J McArdle
Jul 1, 1986·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·W Finger, C Martin
Jun 1, 1983·Naunyn-Schmiedeberg's Archives of Pharmacology·V Dolezal, S Tucek
Nov 4, 1982·Brain Research·R Tapia, M Sitges
Nov 19, 1981·European Journal of Pharmacology·K Enomoto, T Maeno
Oct 22, 1982·European Journal of Pharmacology·N N DurantR L Katz
May 8, 1985·European Journal of Pharmacology·K Enomoto, T Maeno
Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·A R KhanJ Molgo
Nov 1, 1982·Journal of the Autonomic Nervous System·S Stolc, E Vlcková
Aug 15, 2002·International Journal of Pharmaceutics·Stéphane GibaudAlain Astier
Apr 4, 2008·Journal of Neurophysiology·Timothy J Searl, Eugene M Silinsky
Aug 5, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Fujun LuoStephen D Meriney
Aug 31, 2002·Radiology·Takehisa NakaharaJunji Miyakoshi
Oct 16, 2008·Clinical and Experimental Hypertension : CHE·Karim A Alkadhi
Apr 1, 1988·British Journal of Pharmacology·D EstrellaI G Marshall
Jan 22, 1982·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J Molgó, S Thesleff
Jan 1, 1993·Medicinal Research Reviews·A L Harvey
Mar 1, 1983·Acta Pharmacologica Et Toxicologica·R A Khan, M Lemeignan
Nov 1, 1996·Journal of Applied Physiology·E Van Lunteren, M Moyer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.