The effects of a mixture of charged and neutral additives on analyte migration behavior in capillary electrophoresis

Electrophoresis
A R KranackD D Chen

Abstract

Multicomponent additives, such as derivatized cyclodextrins with various degrees of substitution, can be considered single-component additives as long as the fraction of each component remains constant. In this paper, equations are derived describing the effect of such additives on the migration behavior of analytes. These equations are used in the study of capillary electrophoresis (CE) systems with differentially charged cyclodextrins as additives. For weakly acidic analytes, the binding with highly negatively charged sulfobutyl ether beta-cyclodextrin (SBE-beta-CD) increases their negative electrophoretic mobility, while the binding with neutral hydroxypropyl-beta-cyclodextrin (HP-beta-CD) decreases their negative mobility. By obtaining the equilibrium constants and mobilities for each additive with each analyte (in this case, phenol, 2-naphthol and 1-naphthol), the migration behavior of these analytes in CE systems is quantitatively predicted at various concentrations of mixtures of the two additives. The properties of the contour lines in the binding isotherm surfaces of such CE systems are discussed.

References

Oct 18, 1991·Journal of Chromatography·J SnopekI Jelinek
Dec 1, 1967·Chromatographic Reviews·S Hjertén

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Citations

Apr 29, 1998·Electrophoresis·M T Bowser, D D Chen
Jan 28, 2014·Journal of Chromatography. a·Ludmila MüllerováBohuslav Gaš
Apr 19, 2011·International Journal of Pharmaceutics·Alexei MerzlikineTakashi Mano
Aug 26, 2016·Analytical and Bioanalytical Chemistry·Pavel DubskýMartin Ansorge
Sep 20, 2011·Dental Materials : Official Publication of the Academy of Dental Materials·Martino ColonnaLorenzo Breschi
Mar 29, 2002·Electrophoresis·Philip Britz-McKibbin, David D Y Chen

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