The effects of a new tissue plasminogen activator analogue, Fb-Fb-CF, on cerebral reperfusion in a rabbit embolic stroke model

Annals of Neurology
D A PhillipsR H Pang

Abstract

Early fibrinolytic therapy with full molecular tissue plasminogen activator (t-PA) has been observed to be both angiographically and clinically effective when employed in animal stroke models. Preliminary clinical trials with t-PA are in progress. It is possible to refine t-PA by developing fragments or analogues of the drug. Using recombinant DNA technology in the Escherichia coli system, a t-PA analogue consisting of the catalytic fragment of t-PA and a dimer of the B fragment of staphylococcal protein A (Fb-Fb-CF) has been produced. Because this analogue has a long serum half-life of 90 minutes, we employed Fb-Fb-CF in a rabbit cerebral embolic stroke model to assess its efficacy as a reperfusion agent. When given as a bolus to 10 animals 15 minutes after embolization, Fb-Fb-CF produced angiographic cerebral reperfusion in 48 +/- 21 minutes (+/- SD), while in 8 saline-treated controls, reperfusion was not observed at 180 minutes in any animal (p less than 0.01). In another experiment reperfusion was demonstrated at 66 +/- 32 minutes in 11 animals treated with Fb-Fb-CF 90 minutes after embolization as compared with 100 +/- 25 minutes in 12 saline-treated controls (p less than 0.01). A small macroscopic hemorrhage within an in...Continue Reading

References

Sep 1, 1987·Journal of Neurosurgery·S M PapadopoulosJ C Sackellares
Nov 1, 1987·Journal of the American College of Cardiology·D Collen
Nov 1, 1987·Journal of the American College of Cardiology·B E Sobel
Oct 1, 1987·Journal of the American College of Cardiology·E BraunwaldR Solomon
Jan 1, 1983·Annals of Neurology·M E Raichle

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Citations

Apr 16, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·E BuschK A Hossmann
Jan 1, 1992·Progress in Cardiovascular Diseases·S R Levine, T G Brott
Oct 1, 1992·The Journal of Surgical Research·D M WisemanP M Scholz
Apr 27, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·C X WangA Shuaib
Apr 1, 1990·Stroke; a Journal of Cerebral Circulation·D A PhillipsR H Pang
Aug 1, 1992·Stroke; a Journal of Cerebral Circulation·K OvergaardN H Diemer
Jun 1, 1992·Stroke; a Journal of Cerebral Circulation·L P CarterO Temeltas
May 1, 1996·Stroke; a Journal of Cerebral Circulation·K YanakaR C Heros
Feb 1, 1996·Italian Journal of Neurological Sciences·L CandeliseA P Maggioni

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