The Effects of a Pulsed Electromagnetic Field on the Proliferation and Osteogenic Differentiation of Human Adipose-Derived Stem Cells

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Yukun YinJing Tian

Abstract

BACKGROUND A low frequency pulsed electromagnetic field (PEMF) has been confirmed to play an important role in promoting the osteogenic differentiation of human bone marrow stem cells (BMSCs). Adipose-derived stem cells (ASCs) possess some attractive characteristics for clinical application compared to BMSCs, such as abundant stem cells from lipoaspirates, faster growth, less discomfort and morbidity during surgery. ASCs can become adipocytes, osteoblasts, chondrocytes, myocytes, neurocytes, and other cell types. Thus, ASCs might be a good alternative in clinical work involving treatment with PEMF. MATERIAL AND METHODS Human ASCs (hASCs)were divided into a control group (without PEMF exposure) and an experimental group (PEMF for two hours per day). We examined the effect of PEMF on promoting cell proliferation and osteogenic differentiation from several aspects: CCK-8 proliferation assay, RNA extraction, qRT-PCR detection, western blotting, and immunofluorescence staining experiments. RESULTS PEMF could promote cell proliferation of human ASCs (hASCs) at an early stage as determined by CCK-8 assay. A specific intensity (1 mT) and frequency (50 Hz) of PEMF promoted osteogenic differentiation in hASCs in alkaline phosphatase (ALP...Continue Reading

Citations

Oct 28, 2018·Progress in Biomaterials·Nasim RezaeeNooshin Haghighipour
Mar 26, 2019·Bioelectromagnetics·Carlo GalliStefano Guizzardi
Jan 22, 2019·Electromagnetic Biology and Medicine·Esmaeel AzadianAbdolreza Ardeshirylajimi
Sep 27, 2018·International Journal of Biomaterials·Carlo GalliStefano Guizzardi

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BETA
PCR
electrophoresis

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SPSS
ImageJ
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