PMID: 6399244Jan 1, 1984Paper

The effects of activating peptides and proteins on the activity of reconstituted cholesterol desmolase systems

Endocrine Research
N J Greenfield

Abstract

The kinetics of cleavage of both cholesterol and cholesterol sulfate by reconstituted cholesterol side-chain cleavage systems are profoundly modified by non-ionic detergents and phospholipids. The Km value for adrenodoxin is changed depending on whether cholesterol or cholesterol sulfate is used as the substrate and by the presence of detergents and phospholipids in the assay mixture. The activity of the enzyme system is also affected by reducing agents such as dithiothreitol. All these factors must be considered when the effects of protein and peptide activators of the cholesterol side-chain cleavage system are interpreted.

References

Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R B HochbergS Lieberman
Mar 1, 1979·Molecular and Cellular Endocrinology·E R Simpson
Aug 1, 1981·The Journal of Cell Biology·P F HallC L Thomasson
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R C Pedersen, A C Brownie
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P A WarneS Lieberman
Aug 1, 1954·Archives of Biochemistry and Biophysics·D STONE, O HECHTER

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