The effects of aging on biosynthetic processes in the rat hypothalamic osmoregulatory neuroendocrine system

Neurobiology of Aging
Michael P GreenwoodDavid Murphy

Abstract

Elderly people exhibit a diminished capacity to cope with osmotic challenges such as dehydration. We have undertaken a detailed molecular analysis of arginine vasopressin (AVP) biosynthetic processes in the supraoptic nucleus (SON) of the hypothalamus and secretory activity in the posterior pituitary of adult (3 months) and aged (18 months) rats, to provide a comprehensive analysis of age-associated changes to the AVP system. By matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis, we identified differences in pituitary peptides, including AVP, in adult and aged rats under both basal and dehydrated states. In the SON, increased Avp gene transcription, coincided with reduced Avp promoter methylation in aged rats. Based on transcriptome data, we have previously characterized a number of novel dehydration-induced regulatory factors involved in the response of the SON to osmotic cues. We found that some of these increase in expression with age, while dehydration-induced expression of these genes in the SON was attenuated in aged rats. In summary, we show that aging alters the rat AVP system at the genome, transcriptome, and peptidome levels. These alterations however did not affect circulating level...Continue Reading

Citations

Apr 23, 2020·Journal of Neuroendocrinology·Mingkwan GreenwoodMichael Paul Greenwood
Mar 27, 2021·Frontiers in Physiology·Sina StuckerAnjali P Kusumbe
Apr 4, 2021·International Journal of Molecular Sciences·Ming-Hui YangYu-Chang Tyan
Jul 24, 2021·Journal of Neuroendocrinology·Audrys G PaužaMichael P Greenwood
Jul 14, 2021·Anatomy & Cell Biology·Islam Omar Abdel FattahWael Amin Nasr El-Din

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