The effects of anesthesia, mouse strain and age on intraocular pressure and an improved murine model of experimental glaucoma.

Experimental Eye Research
Frances E ConeHarry A Quigley

Abstract

The purpose of this study was to improve a mouse model of chronic intraocular pressure (IOP) elevation utilizing microbead injection in two strains of mice and to assess the effect of age and anesthesia on measured IOP. We compared our previous model with two modified protocols for injecting polystyrene microbeads and viscoelastic material in CD1or C57BL/6 mice. The measured outcomes were degree of IOP elevation and production of axonal loss. The first new protocol was injection of 3 μL of equal volumes of 6 μm and 1 μm diameter beads, followed by 2 μL of viscoelastic (3+2). The second new protocol injected 4 μL of the two bead mixture, then 1 μL of viscoelastic (4+1). Both were compared to injection of 2 μL of 6 μm beads with 3 μL of viscoelastic (2+3). We also compared the effects of age and of two anesthetic regimens (intraperitoneal ketamine/xylazine/acepromazine versus isoflurane gas) on measured IOP in untreated eyes of both strains. IOP was 2mm Hg lower with intraperitoneal than with gas anesthesia in both strains (p=0.003, p<0.0001, t-test). IOP measurements were lower in untreated young (2 months) compared to older (10 months) C57BL/6 mice (p=0.001, t-test). In the experimental glaucoma mouse model, mean IOP and number...Continue Reading

References

Apr 12, 2002·Acta Physiologica Scandinavica·H SchulzR Balling
Feb 26, 2003·Investigative Ophthalmology & Visual Science·John DaniasThom Mittag
Sep 26, 2003·Investigative Ophthalmology & Visual Science·Makoto AiharaRobert N Weinreb
Sep 26, 2003·Investigative Ophthalmology & Visual Science·Sinisa D GrozdanicRandy H Kardon
Aug 17, 2005·PLoS Genetics·Richard T LibbySimon W M John
Nov 29, 2005·Experimental Eye Research·Javier Ruiz-Ederra, A S Verkman
Feb 10, 2006·Experimental Eye Research·Christy A MorrisRobert N Weinreb
Feb 24, 2007·American Journal of Ophthalmology·Dana BlumbergHarry Quigley
Sep 22, 2007·Documenta Ophthalmologica. Advances in Ophthalmology·William R WoodwardDe-Ann M Pillers
Mar 22, 2008·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Thomas V JohnsonCarol B Toris
Dec 25, 2008·Experimental Eye Research·Stuart J McKinnonRobert W Nickells
Oct 10, 2009·Investigative Ophthalmology & Visual Science·Christine T Fu, David Sretavan
Oct 24, 2009·Investigative Ophthalmology & Visual Science·Rebecca M SappingtonDavid J Calkins
Aug 13, 2010·Investigative Ophthalmology & Visual Science·Huihui ChenDong F Chen
Aug 20, 2010·Investigative Ophthalmology & Visual Science·Mary E PeaseHarry A Quigley
Apr 5, 2011·Experimental Eye Research·Chun DingNing Tian

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Citations

Dec 12, 2012·Investigative Ophthalmology & Visual Science·Benjamin J FrankfortSamuel M Wu
Feb 14, 2013·Investigative Ophthalmology & Visual Science·Cathy NguyenHarry A Quigley
Jul 4, 2013·Investigative Ophthalmology & Visual Science·Lakisha K BuieTeresa Borrás
Jul 26, 2014·The Journal of Clinical Investigation·Dae-Young ParkGou Young Koh
May 17, 2014·Current Eye Research·Michaël J A GirardNicholas G Strouthidis
Jun 28, 2015·Experimental Eye Research·James E Morgan, James R Tribble
Aug 2, 2014·Scientific Reports·Adrian SmedowskiJoanna Lewin-Kowalik
Dec 26, 2013·Experimental Eye Research·Matthew R SteinhartHarry A Quigley
Apr 29, 2015·Experimental Eye Research·Derek M NusbaumBenjamin J Frankfort
Mar 31, 2015·Neurobiology of Aging·Henry C TsengMichael D Ehlers
Jun 13, 2015·BioMed Research International·Makoto IshikawaYukitoshi Izumi
Jan 12, 2017·Expert Opinion on Drug Discovery·Renu Agarwal, Puneet Agarwal
Mar 8, 2016·PloS One·Joseph M SherwoodDarryl R Overby
Jan 13, 2018·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Najam A Sharif
Jan 10, 2020·The Journal of Experimental Medicine·Wei LiuWenbo Zhang
Aug 22, 2020·PloS One·Sarah QuillenElizabeth Kimball
Dec 15, 2018·Acta Ophthalmologica·Sayantan Biswas, Kelvin H Wan
Sep 11, 2020·Investigative Ophthalmology & Visual Science·Yik Tung Tracy LingThao D Nguyen
Jun 18, 2017·Frontiers in Neuroscience·Franklin D EchevarriaRebecca M Sappington
Dec 14, 2018·Investigative Ophthalmology & Visual Science·Emily MathieuYeni H Yücel
Apr 9, 2017·Documenta Ophthalmologica. Advances in Ophthalmology·Akshay GurditaVivian Choh
May 28, 2020·Investigative Ophthalmology & Visual Science·Ralph J HazlewoodRachel W Kuchtey
Mar 25, 2019·Frontiers in Neuroscience·Daniel T Hass, Colin J Barnstable
Oct 6, 2020·Frontiers in Pharmacology·Michał FiedorowiczPaweł Grieb
Nov 6, 2020·Investigative Ophthalmology & Visual Science·Diane N SayahMark R Lesk
Dec 17, 2020·Journal of the Royal Society, Interface·Arina KornevaThao D Nguyen
Jan 27, 2021·International Journal of Molecular Sciences·Michal FiedorowiczMario Damiano Toro
Dec 29, 2020·Experimental Eye Research·Shunsuke TsuchiyaKazuhisa Sugiyama
Jan 30, 2021·Translational Vision Science & Technology·James R TribblePete A Williams
Jan 11, 2021·Progress in Retinal and Eye Research·Daniel T Hass, Colin J Barnstable
Aug 18, 2021·Veterinary Ophthalmology·Shin Ae Park, András M Komáromy
Aug 20, 2021·Investigative Ophthalmology & Visual Science·Max K ColbertKevin C Chan
Oct 14, 2021·Investigative Ophthalmology & Visual Science·Maria J RodrigoElena Garcia-Martin

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