PMID: 708734Oct 4, 1978Paper

The effects of anion transport inhibitors on structural transitions in erythrocyte membranes

Biochimica Et Biophysica Acta
J SnowP S Low

Abstract

Red blood cell membranes have been labeled with several covalent and noncovalent inhibitors of anion transport and their heat capacity profiles determined as a function of temperature. Covalent inhibitors include the amino reactive agents 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid, pyridoxal phosphate and 1-fluoro-2,4-dinitro benzene. The non-covalent inhibitors include several well known local anesthetics. The study was underataken in order to identify regions of the membrane involved in anion transport. Covalent modification in all case resulted in a large upward shift of the C transition, which is beleived to involve a localized phospholipid region. Evidence is presented which indicates that Band III protein and this phospholipid region are in close physical proximity on the membrane. Addition of non-covalent inhibitors affects the membrane in either or both of two ways. In some cases, a lowering and broadening of the C transition occurs; in others the B1 and B2 transitions are altered. These latter transitions are beleived to involve both phospholipid and protein, including Band III. These results may indicate that the non-covalent inhibitors produce their inhi...Continue Reading

References

Aug 12, 1976·Nature·A G Lee
Jul 18, 1975·Biochimica Et Biophysica Acta·D PapahadjopoulosG Shepherd
Apr 18, 1972·Biochimica Et Biophysica Acta·D Papahadjopoulos
Sep 1, 1971·The Journal of Membrane Biology·J Poensgen, H Passow

Citations

Oct 1, 1978·Archives of Biochemistry and Biophysics·P S Low, J F Brandts
Jan 1, 1980·Annals of the New York Academy of Sciences·P A Knauf, A Rothstein
Mar 31, 2007·Journal of Biochemical and Biophysical Methods·I T IvanovP Chakaarova
Jan 1, 1983·Annals of the New York Academy of Sciences·A K SolomonA S Verkman
Nov 1, 1990·Journal of Cellular Physiology·P A Kudo, T C Lo
Apr 20, 2011·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·I T IvanovI Zlatanov
Jul 17, 1995·Biochimica Et Biophysica Acta·T Haltia, E Freire
Feb 14, 1995·Biochimica Et Biophysica Acta·T Haltia, E Freire

Related Concepts

Topical anesthetic
Anions
Calorimetry
Dinitrofluorobenzene
Red Cell Ghost
Erythrocytes
Pyridoxal Phosphate

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