The effects of arsenic trioxide on DNA synthesis and genotoxicity in human colon cancer cells.

International Journal of Environmental Research and Public Health
Jacqueline J StevensChristian Rogers

Abstract

Colon cancer is the third leading cause of cancer-related deaths worldwide. Recent studies in our laboratory have demonstrated that arsenic trioxide is cytotoxic in human colon cancer (HT-29), lung (A549) and breast (MCF-7) carcinoma cells. The purpose of the present study is to investigate the effects of arsenic trioxide on DNA synthesis and the possible genotoxic effects on human colon cancer cells. HT-29 cells were cultured according to standard protocol, followed by exposure to various doses (0, 2, 4, 6, 8, 10, and 12 microg/mL) of arsenic trioxide for 24 h. The proliferative response (DNA synthesis) to arsenic trioxide was assessed by [(3)H]thymidine incorporation. The genotoxic effects of arsenic-induced DNA damage in a human colon cancer cell line was evaluated by the alkaline single cell gel electrophoresis. Results indicated that arsenic trioxide affected DNA synthesis in HT-29 cells in a biphasic manner; showing a slight but not significant increase in cell proliferation at lower levels of exposure (2, 4 and 6 microg/mL) followed by a significant inhibition of cell proliferation at higher doses (i.e., 8 and 10 microg/mL). The study also confirmed that arsenic trioxide exposure caused genotoxicity as revealed by the si...Continue Reading

References

Dec 16, 1992·Mutation Research·A N JhaA T Natarajan
Jun 1, 1995·International Journal of Radiation Biology·C R KentG G Steel
Nov 1, 1996·Toxicology and Applied Pharmacology·D R GermolecM I Luster
Jun 24, 1999·Environmental Health Perspectives·C O AbernathyM Waalkes
Mar 29, 2000·Environmental and Molecular Mutagenesis·R R TiceY F Sasaki
Apr 15, 2000·Toxicology and Applied Pharmacology·K J TroubaR L Vorce
May 2, 2001·The Oncologist·N C Munshi
Oct 2, 2001·CA: a Cancer Journal for Clinicians·H Frumkin, M J Thun
May 16, 2002·Seminars in Hematology·Kelly DavisonWilson H Miller
Jun 22, 2002·Toxicology Letters·Michael F Hughes
Oct 16, 2002·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hisham K HamadehDori Germolec
Nov 12, 2002·Environmental Health Perspectives·Zigang Dong
Nov 12, 2002·Environmental Health Perspectives·Miroslav StýbloDavid J Thomas
Oct 31, 2003·Toxicologic Pathology·Paul B TchounwouJose A Centeno
Feb 20, 2004·Molecular and Cellular Biochemistry·Paul B TchounwouAnita K Patlolla
Feb 20, 2004·Molecular and Cellular Biochemistry·Chuanshu HuangXianglin Shi
Jul 28, 2004·Toxicology and Applied Pharmacology·Takahiko YoshidaGui Fan Sun
Aug 4, 2004·Free Radical Biology & Medicine·Tom K Hei, Metka Filipic
Oct 11, 2005·Mutation Research·Anita K Patlolla, Paul B Tchounwou
Apr 1, 2006·Mutation Research·Soile Tapio, Bernd Grosche
Feb 24, 2007·Toxicology and Applied Pharmacology·Catherine B KleinToby G Rossman
Jul 20, 2007·Environmental Health Perspectives·How-Ran Guo
Feb 8, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Elke DoppAlbert W Rettenmeier
Jul 22, 2008·Mutation Research·Pritha GhoshKunal Ray
Nov 22, 2008·Mutation Research·Alex GalanisRaphael Sandaltzopoulos
Feb 20, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jiong HuZhu Chen
May 29, 2009·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Nov 17, 2009·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Emir HenićPer-Anders Mårdh

❮ Previous
Next ❯

Citations

May 20, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Hui-Wen ChiuYing-Jan Wang
Apr 3, 2014·Archives of Toxicology·Elisa BustaffaLucia Migliore
Jun 29, 2016·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Samrat DasSarbani Giri
Nov 24, 2012·Microchemical Journal, Devoted to the Application of Microtechniques in All Branches of Science·Anita K PatlollaJose A Centeno
Jun 3, 2014·Journal of Experimental & Clinical Cancer Research : CR·Sanjay KumarPaul B Tchounwou
Jan 13, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Guangjun JiaoKang Yang
Jul 16, 2013·Molecular and Cellular Endocrinology·Ali ZekriArdeshir Ghavamzadeh
Apr 30, 2015·The Libyan Journal of Medicine·Muhammad Nauman ArshadJawad Nazir
Apr 28, 2018·Bulletin of Environmental Contamination and Toxicology·Oluwatosin Adetola ArojojoyeJeremiah Moyinoluwalogo Afolabi
Aug 10, 2018·Journal of Biochemical and Molecular Toxicology·Sanjay KumarPaul B Tchounwou
Oct 30, 2018·Toxicological Research·Jinia Sinha RoyAshok K Giri
Sep 3, 2019·Current Pharmaceutical Design·Maushmi S KumarMayur C Yergeri
Aug 1, 2014·Molecular Biology Reports·Palanisamy SankarSouvendra Nath Sarkar

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
Assay

Software Mentioned

SAS
Automated Comet Assay Analysis System ( LACAAS )

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Dennis J Goussetis, Leonidas C Platanias
Metal Ions in Biology and Medicine : Proceedings of the ... International Symposium on Metal Ions in Biology and Medicine Held ... = Les Ions Métalliques En Biologie Et En Médecine : ... Symposium International Sur Les Ions Métalliques En Biologie Et En M
Alice M WalkerPaul B Tchounwou
© 2021 Meta ULC. All rights reserved