The effects of betamethasone (BM) on endothelial nitric oxide synthase (eNOS) expression in adult baboon femoral arterial endothelial cells

The Journal of Steroid Biochemistry and Molecular Biology
K AidaX L Wang

Abstract

Glucocorticoids have significant effects on endothelium mediated vascular function throughout life. The baboon model has been used extensively to study cellular responses to glucocorticoids at several stages of the lifespan. Endothelial nitric oxide synthase (eNOS) is a major regulator of endothelium dependent arterial vasodilation. We have previously demonstrated that synthetic glucocorticoids down regulate eNOS in the baboon placenta. We have now conducted studies to determine whether glucocorticoids would alter eNOS expression in adult systemic vascular endothelial cells in this important animal model. We explored this potential mechanism in endothelial cells from femoral arteries of adult baboons at necropsy and cultured to the fourth passage. Endothelial cells were treated with 10-100nM betamethasone for 24h at 37 degrees C. Vascular endothelial growth factor (VEGF) was used as a positive control and medium as negative controls. The role of glucocorticoid receptor mediation in betamethasone-induced eNOS changes was investigated with the glucocorticoid receptor antagonist mifepristone. RNA (real-time quantitative RT-PCR) and protein (ELISA) were extracted and measured for eNOS. Expression and subcellular distribution of glu...Continue Reading

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Citations

Jul 11, 2006·Acta Paediatrica·Anna Wasilewska, Walentyna Zoch-Zwierz
Aug 17, 2010·American Journal of Respiratory and Critical Care Medicine·Adam Wanner, Eliana S Mendes
Nov 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ming-Xiang ZhangXing Li Wang
May 10, 2008·Journal of Applied Physiology·Eliana S MendesAdam Wanner

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