The effects of Bifidobacterium breve on immune mediators and proteome of HT29 cells monolayers

BioMed Research International
Borja SánchezMiguel Gueimonde

Abstract

The use of beneficial microorganisms, the so-called probiotics, to improve human health is gaining popularity. However, not all of the probiotic strains trigger the same responses and they differ in their interaction with the host. In spite of the limited knowledge on mechanisms of action some of the probiotic effects seem to be exerted through maintenance of the gastrointestinal barrier function and modulation of the immune system. In the present work, we have addressed in vitro the response of the intestinal epithelial cell line HT29 to the strain Bifidobacterium breve IPLA20004. In the array of 84 genes involved in inflammation tested, the expression of 12 was modified by the bifidobacteria. The genes of chemokine CXCL6, the chemokine receptor CCR7, and, specially, the complement component C3 were upregulated. Indeed, HT29 cells cocultivated with B. breve produced significantly higher levels of protein C3a. The proteome of HT29 cells showed increased levels of cytokeratin-8 in the presence of B. breve. Altogether, it seems that B. breve IPLA20004 could favor the recruitment of innate immune cells to the mucosa reinforcing, as well as the physical barrier of the intestinal epithelium.

References

Dec 26, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hanne R ChristensenJames J Pestka
Mar 9, 2005·World Journal of Gastroenterology : WJG·Alexander SwidsinskiLaura-P Hale
Apr 22, 2005·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·S Di CaroA Gasbarrini
Jun 11, 2005·World Journal of Gastroenterology : WJG·Jing-Gang LanSimon-Richard Carding
May 23, 2006·International Journal of Food Microbiology·Christian U RiedelBernhard J Eikmanns
Jan 15, 2008·Clinical and Experimental Immunology·M SchleeK Fellermann
Aug 4, 2009·International Immunopharmacology·Rossella PaolilloAntonietta Rizzo
Jan 15, 2010·International Journal of Food Microbiology·Patricia LópezAna Suárez
Jul 29, 2010·Nature Reviews. Gastroenterology & Hepatology·Mélanie G GareauW Allan Walker
Sep 9, 2010·Proceedings of the National Academy of Sciences of the United States of America·Peter van BaarlenMichiel Kleerebezem
Nov 27, 2010·International Journal of Food Microbiology·Silvia ArboleyaMiguel Gueimonde
Aug 6, 2011·European Journal of Pharmacology·Per Brandtzaeg
Jun 16, 2012·Nature·Tanya YatsunenkoJeffrey I Gordon
Sep 14, 2012·Nature·Craig L MaynardCasey T Weaver
Jan 19, 2013·Methods in Molecular Biology·Pamela A Wearsch, Peter Cresswell

❮ Previous
Next ❯

Citations

Mar 24, 2016·Journal of Proteomics·Lorena RuizAbelardo Margolles
Jun 30, 2016·Cells·Florian Geisler, Rudolf E Leube
Sep 15, 2016·Cells·Terhi O HeleniusDiana M Toivola
Dec 20, 2017·Frontiers in Microbiology·Lorena RuizAbelardo Margolles
Jun 2, 2018·BioMed Research International·Md Abul Kalam AzadJie Yin
Jan 4, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Iñaki Robles-VeraJuan Duarte
Nov 6, 2020·The International Journal of Biochemistry & Cell Biology·Lauri PolariDiana M Toivola

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR
Profiler
ELISA
Protein Assay

Software Mentioned

SPSS
ImageMaster Platinum

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.