The effects of Bile Acids on Freshly Isolated Rat Glomeruli and Proximal Tubular Fragments

Toxicology in Vitro : an International Journal Published in Association with BIBRA
B KalerP H Bach

Abstract

The role of bile acids in post-surgical acute renal failure in jaundiced patients is obscure. In this study the effects of 11 bile acids were assessed on freshly isolated rat glomeruli and proximal tubular fragments using de novo protein synthesis and lactate dehydrogenase (LDH) leakage as markers of cytotoxicity. Lithocholic acid inhibited protein synthesis from 5mum, chenodeoxycholic and deoxycholic acid from 50mum (P<0.05). The concentration of hydrophobic bile acids that inhibited protein synthesis by 50% (IC(50)) was 10mum, 75mum and 80mum for lithocholic, chenodeoxycholic and deoxycholic acids, respectively. The glycine and taurine conjugates of these bile acids had no significant effect on de novo protein synthesis up to 200mum. Lithocholic acid (50mum), chenodeoxycholic (200mum) and deoxycholic acids (200mum) caused a significant increase (P<0.05) in LDH leakage. Lithocholic acid also directly inhibited LDH activity above 50mum (P<0.05), whereas chenodeoxycholic acid and deoxycholic acid had no effect on LDH below 500mum, at which concentration they caused a slight increase in activity. The cytotoxic bile acids had no effect on the level of reactive oxygen species in kidney fragments. Hydrophobic bile acids inhibit prot...Continue Reading

References

Jul 1, 1990·Renal Physiology and Biochemistry·Y S ShenH S Fang
Nov 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D M HeumanZ R Vlahcevic
Feb 1, 1989·American Journal of Surgery·R B Wait, K U Kahng
Oct 31, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·B A Israeli, E Bogin
Oct 1, 1974·The American Journal of Digestive Diseases·C D FischerE H Mosbach
Nov 1, 1969·Journal of Colloid and Interface Science·M C Carey, D M Small
Oct 10, 1969·Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine·I MakinoK Mashimo
Dec 1, 1967·The Journal of Clinical Investigation·J D Pstrpw
Apr 1, 1981·The Journal of Clinical Investigation·F A WilsonK J Ullrich
Jan 8, 1981·Biochimica Et Biophysica Acta·P J Lowe, R Coleman
Aug 1, 1981·Analytical Biochemistry·E De VendittisV Bocchini
Feb 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S KrähenbühlJ Reichen
May 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R J SokolK O'Brien
Jun 1, 1993·Gastroenterology·S GüldütunaU Leuschner
Apr 1, 1957·The Journal of Clinical Investigation·D RUDMAN, F E KENDALL
Nov 1, 1964·The American Journal of Physiology·I M WEINERL LACK
Apr 7, 1966·The New England Journal of Medicine·C Topuzlu, W M Stahl

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Citations

Aug 22, 2003·Experimental Dermatology·Eri AokiSeiji Kawana
Jul 11, 1998·The Journal of the Association of Nurses in AIDS Care : JANAC·B J Holtzclaw

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