PMID: 8601850Mar 1, 1996Paper

The effects of burn blister fluid on cultured keratinocytes

The Journal of Trauma
B J ReaganM R Madden

Abstract

Previous studies have suggested that burn blister fluid (BBF) may be detrimental to the healing of the underlying wound bed. In this study, the effects of burn blister fluid on cultured keratinocyte proliferation and differentiation were examined and quantitated using various techniques. At three different concentrations (2%, 10%, 20% in 20% fetal bovine serum/complete culture medium), 19 BBFs were tested in triplicate using 12 populations of cultured keratinocytes. All BBFs were collected from partial thickness burns within 72 hours of injury,. BBF was added on day 4 of the keratinocyte culture. The effect on proliferation and viability was assessed using trypan blue dye exclusion. Multiparameter flow cytometric analysis was used to quantitate population kinetics and cell size distribution. Keratinocyte differentiation was determined using immunohistochemical staining of differentiation markers and quantitation of cornified envelope formation. Relative to control fluid, the BBF caused a variable effect on proliferation, ranging from 67% inhibition to 103% stimulation with an overall 4% inhibition. The range of keratinocyte viability was narrower, with a similar overall 4% reduction. Using flow cytometry to analyze RNA/DNA cont...Continue Reading

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Citations

Mar 29, 2006·Journal of Burn Care & Research : Official Publication of the American Burn Association·Rachael L Sargent
Apr 19, 2006·International Wound Journal·Michel H E Hermans
Oct 9, 2002·Advances in Skin & Wound Care·Reg Richard, R Michael Johnson

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