The Effects of Caffeine on Metabolomic Responses to Muscle Contraction in Rat Skeletal Muscle

Nutrients
Satoshi TsudaTatsuro Egawa

Abstract

Exercise has beneficial effects on our health by stimulating metabolic activation of skeletal muscle contraction. Caffeine is a powerful metabolic stimulant in the skeletal muscle that has ergogenic effects, including enhanced muscle power output and endurance capacity. In the present study, we aim to characterize the metabolic signatures of contracting muscles with or without caffeine stimulation using liquid chromatography-mass spectrometry and capillary electrophoresis coupled to mass spectrometry. Isolated rat epitrochlearis muscle was incubated in the presence or absence or of 3 mM caffeine for 30 min. Electrical stimulation (ES) was used to induce tetanic contractions during the final 10 min of incubation. Principal component analysis and hierarchical clustering analysis detected 184 distinct metabolites across three experimental groups-basal, ES, and ES with caffeine (ES + C). Significance Analysis of Microarray identified a total of 50 metabolites with significant changes in expression, and 23 metabolites significantly changed between the ES and ES + C groups. Changes were observed in metabolite levels of various metabolic pathways, including the pentose phosphate, nucleotide synthesis, β-oxidation, tricarboxylic acid c...Continue Reading

References

Sep 1, 1988·Acta Physiologica Scandinavica·N ZetanJ Henriksson
Feb 1, 1987·The Journal of Physiology·M Konishi, S Kurihara
May 1, 1969·The Journal of Experimental Zoology·H Huddart, R G Abram
Jan 1, 1968·The Journal of Physiology·H C Lüttgau, H Oetliker
Sep 1, 1968·Acta Physiologica Scandinavica·B AhlborgC G Nilsson
Dec 1, 1980·The American Journal of Physiology·R NesherD M Kipnis
Jul 10, 2001·American Journal of Physiology. Cell Physiology·R RossiC Reggiani
Oct 5, 2001·Sports Medicine·T E Graham
Nov 21, 2002·Biochemical Society Transactions·D G Hardie, D A Pan
Jan 10, 2003·Food Additives and Contaminants·P NawrotM Feeley
Apr 15, 2004·Medical Hypotheses·Stephen L DoddJohn A St Cyr
Jun 1, 2005·BMC Bioinformatics·Ola LarssonJames A Timmons
Dec 24, 2005·Physiological Reviews·Bente Kiens
Apr 5, 2007·American Journal of Physiology. Endocrinology and Metabolism·Thomas E JensenErik A Richter
Dec 18, 2007·Sports Medicine·François Trudeau
Mar 12, 2009·Current Opinion in Lipidology·Carles Cantó, Johan Auwerx
Feb 6, 2010·Journal of Applied Physiology·Pengxiang SheChristopher J Lynch
Apr 3, 2012·Acta Physiologica·J S V LallyA Bonen
Apr 5, 2012·Nature Reviews. Endocrinology·Bente K Pedersen, Mark A Febbraio
Dec 22, 2012·Diabetes·Marieke G SchoonemanMaarten R Soeters
Oct 4, 2013·American Journal of Physiology. Cell Physiology·Licht MiyamotoTatsuya Hayashi
Sep 23, 2014·Biological Reviews of the Cambridge Philosophical Society·Anna StinconeMarkus Ralser
Jul 15, 2015·Cell Metabolism·Sarah E SeilerDeborah M Muoio
Nov 9, 2016·Journal of Basic and Clinical Physiology and Pharmacology·Eynav HarpazYitzhak Weinstein
Jan 28, 2017·Journal of Animal Science and Biotechnology·Shihai ZhangShiyan Qiao
Aug 23, 2017·Annual Review of Nutrition·Giuseppe GrossoEdward L Giovannucci
May 22, 2018·Advances in Bioscience and Clinical Medicine·Diane E MahoneyJanet D Pierce

❮ Previous
Next ❯

Citations

Jan 8, 2020·Nutrients·Juan Del CosoBeatriz Lara

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
feature extraction
PCA

Software Mentioned

MetaboAnalyst
SAM

Related Concepts

Related Feeds

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.