The effects of calcitonin gene-related peptide on tracheal smooth muscle of guinea-pigs in vitro

British Journal of Pharmacology
H NinomiyaS Hasegawa

Abstract

1. The effect of calcitonin gene-related peptide (CGRP) on airway smooth muscle is controversial. The aim of this study was to determine whether the action of CGRP on tracheal strips of guinea-pigs is modulated by epithelium and whether this peptide-induced action involves other mediators including nitric oxide (NO) and endothelin (ET)-1. 2. CGRP produced a weak dose-dependent increase in guinea-pig tracheal tension in vitro (-logEC50 = 8.5 +/- 0.1, maximum contraction = 8.3 +/- 1.2% of 50 mM KCl-induced contraction, n = 6). In epithelium-depleted preparations, CGRP (10(-7) M)-induced contraction was significantly potentiated from 9.0 +/- 1.9% to 41.1 +/- 6.0% (n = 6). 3. L-NG-nitro-arginine methyl ester (L-NAME, 10(-4) M), which inhibits NO synthesis, enhanced the contractile response to CGRP from 9.0 +/- 1.9% to 31.2 +/- 1.1% (n = 6). Indomethacin (10(-5) M) also enhanced the response to CGRP, although the effect was weak (13.4 +/- 3.2%, n = 6). 4. Anti-ET-1 serum changed the CGRP-induced contraction into a relaxation. After incubation of the trachea with ET-1 (10(-7) M) to attenuate ET-1-induced responses, the CGRP-induced contraction also changed into a relaxation. BQ-123 (an ETA receptor antagonist) and BQ-788 (an ETB rece...Continue Reading

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Citations

Sep 24, 1998·Trends in Pharmacological Sciences·G Folkerts, F P Nijkamp
May 17, 2003·Pulmonary Pharmacology & Therapeutics·Jochen SpringerDavid A Groneberg
Jan 30, 2003·Thorax·F L M Ricciardolo
Nov 17, 1998·American Journal of Respiratory and Critical Care Medicine·P J Barnes
Jan 5, 1999·American Journal of Respiratory and Critical Care Medicine·A CadieuxC Lanoue
Dec 1, 2015·Pulmonary Medicine·Dipanjan BhattacharjeeRahul Magazine
Mar 23, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J F CunhaN Paulino
Jul 23, 2004·Physiological Reviews·Fabio L M RicciardoloGert Folkerts
May 22, 2002·Journal of Cardiovascular Pharmacology·Jwu-Lai YehIng-Jun Chen

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