PMID: 9446699Apr 4, 1998Paper

The effects of changing intracellular pH on calcium and potassium currents in smooth muscle cells from the guinea-pig ureter

Pflügers Archiv : European journal of physiology
R D SmithS Wray

Abstract

Guinea-pig ureteric smooth muscle is unusual in that intracellular acidification increases and alkalinization decreases force production. To help elucidate the mechanism underlying these effects on force we have investigated the effects of changing intracellular pH on both calcium and potassium currents in single cells isolated from the guinea-pig ureter to determine their possible role in force development. Depolarization to +40 mV resulted in a fast transient outward current which was inhibited by 4-aminopyridine but not tetraethylammonium. Intracellular alkalinization (20 mM trimethylamine) increased this current to 179 +/- 24% of the control and resulted in the development of a slowly activating large outward current which was inhibited by tetraethylammonium and washout. Acidification (40 mM sodium butyrate) decreased the fast transient outward current to 58 +/- 3% of the control and did not produce a slowly activating current. When potassium was replaced by caesium in the pipette solution, depolarization to 0 mV resulted in an inward calcium current which was abolished by nifedipine. Intracellular alkalinization increased this current to 126 +/- 11% of the control whereas acidification had the opposite effect, decreasing i...Continue Reading

Citations

Dec 1, 2010·Surgical Oncology·Ralf CzymekPhilipp Hildebrand
Jan 5, 2011·The Journal of Surgical Research·Ralf CzymekAndreas Lubienski
Oct 11, 2008·PLoS Computational Biology·Steven A PrescottTerrence J Sejnowski
Jan 23, 1999·Bioelectromagnetics·Y YenC K Chou
Aug 25, 2006·American Journal of Physiology. Cell Physiology·Jerod S DentonJ C Leiter
Nov 13, 2002·American Journal of Physiology. Heart and Circulatory Physiology·R D SmithSusan Wray
Feb 2, 2006·The Journal of Experimental Biology·Marion Christine HöpflingerStefan Galler

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