The effects of chaperones and the influence of protein assembly on peroxisomal protein import.

The Journal of Biological Chemistry
W J Crookes, L J Olsen

Abstract

Peroxisomal proteins are synthesized in the cytoplasm and post-translationally translocated into the organelle. The role of chaperones and protein folding in peroxisomal protein transport is still unclear. Translocation of proteins into mitochondria requires that precursor proteins assume an extended conformation; cytosolic chaperones are thought to help maintain this conformation. In contrast, peroxisomal protein import does not require unfolding of the targeted protein. However, the molecular chaperones Hsp70 and Hsp40 may be important for translocation. We present several lines of evidence that show that plant peroxisomal protein import is enhanced by chaperones. First, peroxisomes isolated from heat-shocked pumpkin seedling tissues exhibited increased protein import relative to control peroxisomes. Second, antibodies raised against wheat germ cytosolic Hsp70 and Escherichia coli Hsp90 inhibited import of the peroxisomal protein isocitrate lyase. To our knowledge, this is the first time that Hsp90 has been directly implicated in a protein transport event. Third, peroxisomal proteins were immunoprecipitated by wheat germ Hsp70 antibodies. We also present results that suggest that the efficiency of peroxisomal protein import i...Continue Reading

References

Sep 1, 1992·Cell Biochemistry and Function·H Kindl
May 1, 1989·The Journal of Cell Biology·S J GouldS Subramani
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L A AllenC R Raetz
Apr 15, 1974·Journal of Molecular Biology·A TissièresU M Tracy
Jan 1, 1982·Annals of the New York Academy of Sciences·P B LazarowL Wong
Jun 1, 1995·Molecular Biology of the Cell·P A WaltonS Subramani
Jul 21, 1995·The Journal of Biological Chemistry·I J van der KleiM Veenhuis
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y Xu, S Lindquist
Mar 31, 1995·The Journal of Biological Chemistry·M FransenP P Van Veldhoven
May 1, 1994·Trends in Biochemical Sciences·U Jakob, J Buchner
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·J R GloverR A Rachubinski
Dec 1, 1994·The Journal of Cell Biology·J A McNew, J M Goodman
Jun 1, 1994·The Journal of Cell Biology·P A WaltonW J Welch
Jan 15, 1994·European Journal of Biochemistry·R Preisig-MüllerH Kindl
Feb 1, 1993·The Journal of Cell Biology·M Wendland, S Subramani
Mar 15, 1996·Science·G Schatz, B Dobberstein
Aug 1, 1996·Current Opinion in Cell Biology·S Subramani
Jan 1, 1995·Cold Spring Harbor Symposia on Quantitative Biology·M J GethingJ Sambrook
Oct 1, 1996·Plant Molecular Biology·R S BostonE Vierling
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·B WimmerC Gietl
Jun 1, 1995·Trends in Cell Biology·J C Gray, P E Row
Mar 1, 1997·Trends in Cell Biology·V Haucke, G Schatz

❮ Previous
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Citations

Feb 13, 2001·Microscopy Research and Technique·M VeenhuisI J Van Der Klei
Oct 8, 1999·Trends in Cell Biology·S A Teter, D J Klionsky
Feb 15, 2001·Trends in Plant Science·W B PrattL J Olsen
May 30, 2001·Trends in Plant Science·R T MullenR N Trelease
May 2, 2001·Nature Reviews. Molecular Cell Biology·V I Titorenko, R A Rachubinski
Nov 23, 2013·Nature Reviews. Molecular Cell Biology·Jennifer J Smith, John D Aitchison
Nov 1, 2001·Annual Review of Cell and Developmental Biology·P E Purdue, P B Lazarow
Jan 4, 2007·International Journal of Biological Sciences·Guang-Rui LuoWei-Dong Le
Nov 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Koh AokiWilliam J Lucas
Feb 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·T SoellickP H Schreier
Apr 4, 2001·Traffic·J E Legakis, S R Terlecky
Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·Akane KamigakiMuneharu Esaka
May 15, 2003·Molecular Membrane Biology·John A ParkesAlison Baker
Feb 6, 2002·The Journal of Cell Biology·Suresh Subramani
Feb 15, 2015·Journal of Molecular Biology·P K Kim, E H Hettema
Jul 4, 2012·Traffic·Hidenori Otera, Yukio Fujiki
Feb 13, 2001·Experimental Cell Research·S R TerleckyS Subramani
Sep 27, 2015·Biochimica Et Biophysica Acta·Ana F DiasJorge E Azevedo
Feb 1, 2003·Traffic·Cécile B BrocardPaul A Walton
Apr 10, 2015·Open Biology·Marta O FreitasJorge E Azevedo
Aug 14, 1999·Biochimica Et Biophysica Acta·E H HettemaH F Tabak
Jan 30, 2008·Current Protocols in Cell Biology·Nicola Harrison-Lowe, Laura J Olsen
Jul 30, 2003·The Journal of Biological Chemistry·Marcia E OliveiraJorge E Azevedo
Nov 18, 2018·The FEBS Journal·Aurora Barros-BarbosaJorge E Azevedo
May 13, 2003·Microscopy Research and Technique·Marten VeenhuisIda J Van Der Klei
Feb 24, 2001·Traffic·S R Terlecky, M Fransen
Jan 1, 2009·The Arabidopsis Book·Navneet KaurJianping Hu
Sep 19, 2007·The Journal of Biological Chemistry·Jurre HagemanHarm H Kampinga
Feb 21, 2002·Biochimica Et Biophysica Acta·Mary Q StewartJoel M Goodman
Jun 28, 2001·Biochimica Et Biophysica Acta·F A Agarraberes, J F Dice
May 26, 2004·Cellular Signalling·William B PrattDonald B DeFranco

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