PMID: 1543714Jan 31, 1992

The effects of cholesterol oxidation products in sickle and normal red blood cell membranes

Biochimica Et Biophysica Acta
O KucukD A Gage


The oxysterol content in normal and sickle red blood cell (RBC) membranes was assessed using thin-layer chromatography and capillary gas chromatography/mass spectrometry. Several more oxysterols were present in sickle RBCs compared to normal RBCs. Sickle RBC membranes had a higher concentration of 5 alpha,6 alpha-epoxycholesterol, 5 alpha-cholestane-3 beta,5,6 beta-triol, 7-ketocholesterol and 19-hydroxycholesterol than normal RBC membranes. The increased oxysterols in sickle RBC may be an effect of the increased oxidative stress which occurs in sickle RBC membranes. Physical characteristics of normal and sickle RBC membrane ghosts with and without inserted oxysterols were examined by Fourier transform infrared spectroscopy. The data are consistent with a greater sterol content in sickle cells compared to normal RBC membranes, and a possible oxysterol-cholesterol synergism.


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Related Concepts

Thin Layer Chromatography
Fourier Transform
Chromatography, Gas-Liquid-Mass Spectrometry
Sickle Cell Trait
Infrared Spectrophotometry

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