PMID: 1543714Jan 31, 1992

The effects of cholesterol oxidation products in sickle and normal red blood cell membranes

Biochimica Et Biophysica Acta
O KucukD A Gage

Abstract

The oxysterol content in normal and sickle red blood cell (RBC) membranes was assessed using thin-layer chromatography and capillary gas chromatography/mass spectrometry. Several more oxysterols were present in sickle RBCs compared to normal RBCs. Sickle RBC membranes had a higher concentration of 5 alpha,6 alpha-epoxycholesterol, 5 alpha-cholestane-3 beta,5,6 beta-triol, 7-ketocholesterol and 19-hydroxycholesterol than normal RBC membranes. The increased oxysterols in sickle RBC may be an effect of the increased oxidative stress which occurs in sickle RBC membranes. Physical characteristics of normal and sickle RBC membrane ghosts with and without inserted oxysterols were examined by Fourier transform infrared spectroscopy. The data are consistent with a greater sterol content in sickle cells compared to normal RBC membranes, and a possible oxysterol-cholesterol synergism.

References

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Citations

Sep 1, 1994·Lipids·O KüçükM P Westerman
Oct 31, 1991·Biochemical and Biophysical Research Communications·R SzostekM P Westerman
Mar 22, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Wai C YoongAnthony E Michael
Jan 9, 2007·Redox Report : Communications in Free Radical Research·David M van ReykWendy Jessup
Jul 1, 1993·Medicinal Research Reviews·Y BarenholzA Gabizon
Jun 13, 2019·Scandinavian Journal of Clinical and Laboratory Investigation·Ahmet YalcinkayaYesim Oztas
Jul 14, 1998·American Journal of Hematology·M P WestermanL J Lis
Apr 1, 1993·American Journal of Hematology·O KucukM P Westerman
Dec 16, 1998·Redox Report : Communications in Free Radical Research·A C CarrC C Winterbourn
Jan 5, 2000·Physiological Reviews·G J Schroepfer
Oct 22, 2019·Biochimica Et Biophysica Acta. Biomembranes·Marta Targosz-KoreckaPatrycja Dynarowicz-Latka
Jan 14, 2020·Redox Biology·Amelia AndersonMatthew S O'Connor

Related Concepts

Epicholesterol
Thin Layer Chromatography
Erythrocytes
Fourier Transform
Chromatography, Gas-Liquid-Mass Spectrometry
Oxidation-Reduction
Sickle Cell Trait
Infrared Spectrophotometry

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