The effects of chromium(III) coordination on the dissociation of acidic peptides

Journal of Mass Spectrometry : JMS
Dan PuC J Cassady

Abstract

The complexes formed between chromium(III) and synthetic acidic peptides were studied by sustained off-resonance irradiation collision-induced dissociation (SORI-CID) in a Fourier transform ion-cyclotron resonance (FT-ICR) mass spectrometer equipped with electrospray ionization (ESI). Neutral peptides and peptides containing one, two, and multiple acidic residues were studied. Formation of [M + Cr-2H]+ occurred for all peptides. Three noteworthy features were found in the CID spectra of [M + Cr-2H]+. The first is that fewer fragment ions were produced from [M + Cr-2H]+ than from [M + H]+. The reason may be that multiple coordination between chromium(III) and carboxylate or carbonyl groups hinders the production of fragment ions by continuing to bind pieces of the peptide to chromium(III) after cleavage of bonds within the peptide. The second feature is loss of CO from [M + Cr-2H]+ and [y(n) + Cr-H]+. A mechanism involving coordination of chromium(III) with carboxylate groups is proposed to rationalize elimination of CO. The third feature is that chromium(III) is retained in all fragment ions, indicating strong binding of the metal ion to the peptides. The complex [M + 2Cr-5H]+ is formed as the peptide chain length and number of...Continue Reading

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Citations

Jan 4, 2013·Journal of the American Society for Mass Spectrometry·Tawnya G FlickEvan R Williams
Aug 3, 2013·Journal of the American Society for Mass Spectrometry·Laura BanuDiethard K Bohme
Nov 30, 2011·Journal of Mass Spectrometry : JMS·Heather M WatsonCarolyn J Cassady
Jun 14, 2016·Journal of the American Society for Mass Spectrometry·Juliette J Commodore, Carolyn J Cassady
Feb 9, 2017·Journal of Mass Spectrometry : JMS·Juliette J Commodore, Carolyn J Cassady
May 30, 2014·The American Journal of Chinese Medicine·Chao-Yun WangChun-Hua Wang
Dec 3, 2009·The Journal of Physical Chemistry. a·James S PrellEvan R Williams

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