The effects of ciliary neurotrophic factor on murine spinal cord neurons subjected to dendrite transection injury

Brain Research
L J Rosenberg, J H Lucas

Abstract

Ciliary neurotrophic factor (CNTF) has been found to increase neuronal survival during development and after axotomy. The present study tested the effects of CNTF on lesioned and uninjured mouse spinal cord (SC) neurons grown in tissue culture. An initial toxicity study found that a 24-72 h exposure of SC cultures to concentrations of CNTF above 1000 ng/ml caused stress and death of unlesioned neurons and glia. Pre-selected SC neurons were then subjected to transection of a primary dendrite 100 microns from the edge of the perikaryon (approximately 50% average survival at 24 h). Application of CNTF at concentrations ranging from 0.5 to 1000 ng/ml immediately after lesioning had no statistically significant effects on SC neuron survival 24 h after dendrotomy. Separation of control (no CNTF) and CNTF-treated cells into groups of putative alpha-motor (multipolar with somal diameters > or = 25 microns) and non-alpha-motor neurons (< 25 microns somal diameters) also failed to reveal any significant differences in survival. The lack of protection by CNTF of lesioned SC neurons in mature (21-28 DIV) cultures may reflect a loss of sensitivity to CNTF that occurs with development. Alternatively, protection by CNTF may require co-factors...Continue Reading

Citations

May 24, 2007·Journal of Neurotrauma·Benjamin S ElkinBarclay Morrison
Oct 23, 2019·Advances in Wound Care·Pinghui ZhouWenguo Cui
Dec 14, 2004·Current Eye Research·Kazuhito IkedaChikao Nakayama

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.