The Effects of Co-transplantation of Olfactory Ensheathing Cells and Schwann Cells on Local Inflammation Environment in the Contused Spinal Cord of Rats

Molecular Neurobiology
Jie-yuan ZhangBing-cang Li

Abstract

Inflammatory response following spinal cord injury (SCI) is important in regulation of the repair process. Olfactory ensheathing cells (OECs) and Schwann cells (SCs) are important donor cells for repairing SCI in different animal models. However, synergistic or complementary effects of co-transplantation of both cells for this purpose have not been extensively investigated. In the present study, we investigated the effects of co-transplantation of OECs and SCs on expression of pro- or anti-inflammatory factor and polarization of macrophages in the injured spinal cord of rats. Mixed cell suspensions containing OECs and SCs were transplanted into the injured site at 7 days after contusion at the vertebral T10 level. Compared with the DMEM, SC, or OEC group, the co-transplantation group had a more extensive distribution of the grafted cells and significantly reduced number of astrocytes, microglia/macrophage infiltration, and expression of chemokines (CCL2 and CCL3) at the injured site. The co-transplantation group also significantly increased arginase+/CD206+ macrophages (IL-4) and decreased iNOS+/CD16/32+ macrophages (IFN-γ), which was followed by higher IL-10 and IL-13 and lower IL-6 and TNF-α in their expression levels, a smal...Continue Reading

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Citations

Jun 16, 2016·F1000Research·Christopher S AhujaMichael Fehlings
Jan 6, 2019·Journal of Neurosurgery. Spine·Jetan H BadhiwalaMichael G Fehlings
Feb 27, 2020·Nature Reviews. Neurology·Susan L LindsaySusan C Barnett
Jul 22, 2020·Stem Cells Translational Medicine·Christopher S AhujaMichael G Fehlings
Nov 23, 2017·BioMed Research International·Guan Zhang, Ping Yang
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Aug 30, 2018·International Journal of Molecular Sciences·Damien D PearseMousumi Ghosh
Feb 15, 2020·Journal of Neurotrauma·Aaron D GilmourJames A St John
Oct 18, 2020·International Journal of Molecular Sciences·Anam AnjumYogeswaran Lokanathan
Apr 23, 2020·Current Stem Cell Research & Therapy·Jiawei ShuFangcai Li
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Lynn NazarethJenny Ekberg

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Methods Mentioned

BETA
FCS
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

SPSS
Image Pro Plus
Adobe Photoshop
Quantity One

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