The Effects of Continuous In Vivo Administration of Nisin on Staphylococcus aureus Infection and Immune Response in Mice

Probiotics and Antimicrobial Proteins
A M BrandL M T Dicks

Abstract

Mice were intraperitoneally infected with 2 × 10(8) cfu Staphylococcus aureus Xen 36 and treated with 2,130 AU (arbitrary units) nisin (equivalent to 27.7 μg pure nisin), a class Ia lantibiotic, over 7 days. The metabolic activity of S. aureus Xen 36, concluded from changes in cell bioluminescence, declined for the first 3.5 h, but increased over the next 24 h and remained at this level for the remainder of the 7-day trial. Similar results were obtained with heat-inactivated (25 min at 121 °C) nisin, suggesting that the decline in metabolic activity of S. aureus Xen 36 cannot be attributed to the bacteriostatic activity of nisin. The decline in lymphocyte numbers in infected mice was of smaller magnitude after treatment with active nisin compared to inactive nisin, suggesting that active nisin limited the apoptosis of lymphocytes. The drastic increase in neutrophil versus lymphocyte (N:L) ratio observed in the presence of active nisin suggested that the decline in metabolic activity of S. aureus Xen 36 was due to an immune response triggered by the infection. Nisin, active or inactive, stimulated the activity of cytokines interleukin-6, interleukin-10 and tumour necrosis factor. However, the overall immune response triggered by...Continue Reading

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Citations

Jan 27, 2019·American Journal of Reproductive Immunology : AJRI·Zhifeng JiaBo Liu
Jul 10, 2019·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Michelle V MouritzenHåvard Jenssen
Mar 28, 2019·Journal of Animal Physiology and Animal Nutrition·Joanna MałaczewskaAndrzej K Siwicki
Sep 26, 2020·Frontiers in Microbiology·Santosh Kumar TiwariMichael L Chikindas
Jan 7, 2021·Probiotics and Antimicrobial Proteins·Haodan ZhuKongwang He
Dec 29, 2020·Critical Reviews in Food Science and Nutrition·Silpa S, Rupachandra S
Dec 29, 2020·Peptides·Joanna Małaczewska, Edyta Kaczorek-Łukowska

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