The effects of cutting solutions on the viability of GABAergic interneurons in cerebral cortical slices of adult mice

Journal of Neuroscience Methods
Yasuyo TanakaTakeshi Kaneko

Abstract

To obtain viable GABAergic interneurons in cerebral cortical slices of adult mice, we investigated the effects of slice cutting solutions on the viability of green fluorescent protein (GFP)-expressing cortical neurons in GAD67-GFP knock-in mice. Almost no nuclei of GFP-positive neurons were labeled with propidium iodide in incubated slices, suggesting that GFP fluorescence was a useful indicator for the viability of GABAergic interneurons. When several cutting solutions were compared with saline-based solution, N-methyl-d-glucamine-based sodium-free solution was most effective to keep the number of GFP-positive neurons near the level of perfusion-fixed brain. GFP-positive neurons in slices cut with sodium-free solution were more numerous in cortical layers V-VI, at 30-60 microm depth from the cut surface and 1-6h after cutting than those with saline-based solution. Furthermore, the number of GFP-positive neurons decreased in the cutting condition of high calcium concentration (5mM) or high temperature (37 degrees C), and GFP fluorescence decreased when cut at 0 degrees C. The present results indicate that cutting the brain at 20 degrees C in sodium-free solution is a method for preparing cortical slices with GABAergic interneur...Continue Reading

References

Sep 1, 1989·Pflügers Archiv : European journal of physiology·F A EdwardsT Takahashi
Jun 1, 1995·Journal of Neuroscience Methods·P G AitkenK L Panizzon
Jun 1, 1995·Journal of Neuroscience Methods·P LiptonP M Larkman
May 1, 1997·Canadian Journal of Physiology and Pharmacology·D A PrinceI Parada
Sep 1, 1997·Cerebral Cortex·Y Kawaguchi, Y Kubota
Feb 25, 1998·Brain Research. Brain Research Protocols·R MonetteP Morley
Apr 12, 2000·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·R Sattler, M Tymianski
Mar 5, 2002·Nature·Henriette van PraagFred H Gage
Jul 9, 2002·Journal of Neuroscience Methods·Raad NashmiMichael G Fehlings
Sep 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mario Galarreta, Shaul Hestrin
Nov 15, 2002·Brain Research. Brain Research Protocols·Corinne BranaLars Sundstrom
Apr 27, 2004·Nature·Christoph Schmidt-HieberJosef Bischofberger
Sep 21, 2004·Nature Reviews. Neuroscience·Henry MarkramCaizhi Wu
Jan 25, 2005·Neuron·Anthony J G D HoltmaatKarel Svoboda
Mar 2, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Antoine M ValeraPhilippe Isope

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Citations

Oct 5, 2010·Journal of Neurophysiology·Deepti RaoPaul B Manis
May 29, 2012·Journal of Neurosurgery·Chris KaoPeter E Konrad
Jun 2, 2014·Nature Neuroscience·Yoshito MasamizuMasanori Matsuzaki
Jun 4, 2015·Journal of Clinical Neurophysiology : Official Publication of the American Electroencephalographic Society·Liset Menendez de la PridaJoshua A Dian
Dec 26, 2013·Experimental Neurology·Marius EickhoffAli Gorji
Oct 25, 2016·Neuroscience Research·Hiroyuki OhtaHiromu Yawo
Jan 10, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Risa TamuraMasashi Nibuya
Dec 24, 2017·The Journal of Physiology·Andrew S Bohannon, John J Hablitz
Oct 28, 2011·Journal of Neurophysiology·Pratip Mitra, Robert M Brownstone
Jul 19, 2013·Journal of Neurophysiology·Asher J AlbertsonJohn J Hablitz
Feb 15, 2018·Frontiers in Cellular Neuroscience·Andrew S Bohannon, John J Hablitz
Sep 26, 2019·Frontiers in Synaptic Neuroscience·Aundrea F BartleyLynn E Dobrunz
May 5, 2017·Frontiers in Cellular Neuroscience·Asher J AlbertsonJohn J Hablitz
May 28, 2021·Nature·Kenta M HagiharaAndrew Holmes

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