PMID: 8944329Jan 1, 1996Paper

The effects of cyclosporin A, tamoxifen, and medroxyprogesterone acetate on the enhancement of adriamycin cytotoxicity in primary cultures of human breast epithelial cells

Breast Cancer Research and Treatment
J A Claudio, J T Emerman

Abstract

Adriamycin (Adr), the single most active agent used in the treatment of breast cancer, may become ineffective as treatment progresses due to the development of multidrug resistant (MDR) tumors. A major mechanism associated with MDR is increased P-glycoprotein (Pgp) expression. This study examined the abilities of the anti-estrogen tamoxifen (TAM) and the progestin medroxyprogesterone acetate (MPA) as well as cyclosporin A (CsA), a known resistance modifier, to enhance the cytotoxic effects of Adr on human breast epithelial cells (HBEC) in primary culture. Pgp and estrogen receptor (ER) expression were determined in each of the cultures by immunocytochemical assays using the monoclonal antibodies C219 and H222 Sp gamma, respectively. The Adr-sensitive, Pgp-, ER+ MCF-7 cell line and the Adr-resistant, Pgp+, ER- MCF7-AdrR cell line were used as controls. Primary cultures were categorized as HBEC from tissues with or without previous chemotherapy. Pgp was detected in 1 of the 15 cell cultures from tissues without previous chemotherapy and in 5 of the 6 cell cultures from tissues previously exposed to chemotherapy. Incubation with either CsA or MPA plus Adr enhanced Adr toxicity in Pgp+ but not Pgp- cell cultures, whereas TAM had no...Continue Reading

References

Sep 9, 1992·International Journal of Cancer. Journal International Du Cancer·R D WhelanB T Hill
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·O W PetersenM J Bissell
Sep 1, 1992·The Journal of Clinical Endocrinology and Metabolism·M Thomas, J D Monet
Jan 1, 1991·Cancer Chemotherapy and Pharmacology·P R Twentyman, K A Wright
Jun 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S L SchneiderW L McGuire
Dec 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J T Emerman, D A Wilkinson
Jan 1, 1990·Cancer Chemotherapy and Pharmacology·J T EmermanP M Rebbeck
Apr 15, 1989·International Journal of Cancer. Journal International Du Cancer·N A ShaikhH Braunsberg
Feb 15, 1989·Biochemical and Biophysical Research Communications·M NaitoT Tsuruo
May 3, 1989·Journal of the National Cancer Institute·S E SalmonW S Dalton
Dec 1, 1989·British Journal of Cancer·J SchneiderM Volm
Feb 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J T EmermanP M Rebbeck
Aug 1, 1988·Annals of Internal Medicine·S S Legha
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S VeneroniR Silvestrini

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Citations

Jul 13, 2005·Transplant International : Official Journal of the European Society for Organ Transplantation·Pehr RisslerLennart C Eriksson
Nov 6, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Véronique MargueriteMarc Merten
May 18, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Eleftherios P MamounasNorman Wolmark
Jul 21, 2004·The Journal of Biological Chemistry·Edward Norris-CervettoTerry D Butters

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