The Effects of Dabigatran and Rivaroxaban on Markers of Polymorphonuclear Leukocyte Activation

Pharmaceuticals
Guy A RichardsRonald Anderson

Abstract

Dabigatran is an oral direct thrombin inhibitor, and rivaroxaban, a factor Xa inhibitor. Dabigatran has been implicated in the etiology of acute coronary syndromes and as these occur following inflammatory changes in the endothelium, we investigated the inflammatory potential of these agents in vitro. In order to do so, polymorphonuclear leukocytes (PMNL) were isolated from heparinized venous blood from non-smoking, healthy adults and exposed to dabigatran or rivaroxaban (0.5⁻10 µM). Generation of reactive oxygen species (ROS), elastase release, cytosolic Ca2+ fluxes, neutrophil extracellular trap (NET) formation and cell viability were measured using chemiluminescence, spectrophotometric and flow cytometric procedures respectively. However, with the exception of modest inhibitory effects on elastase release, neither agent at concentrations of up to 10 µM affected these markers of PMNL activation. Although no pro-inflammatory effects of dabigatran nor any difference between the two test agents were detected in vitro, the existence of a pro-inflammatory mechanism involving the generation of thrombin during dabigatran therapy cannot be fully excluded.

References

Jan 14, 1999·The New England Journal of Medicine·R Ross
Aug 17, 2005·Journal of Thrombosis and Haemostasis : JTH·J I Weitz, S M Bates
Sep 1, 2009·The New England Journal of Medicine·Stuart J ConnollyUNKNOWN RE-LY Steering Committee and Investigators
Dec 8, 2009·The New England Journal of Medicine·Sam SchulmanUNKNOWN RE-COVER Study Group
Nov 5, 2010·The New England Journal of Medicine·Stuart J ConnollyUNKNOWN Randomized Evaluation of Long-Term Anticoagulation Therapy Investigators
Oct 20, 2011·British Medical Bulletin·Mohammad Madjid, James T Willerson
Jul 3, 2013·International Immunopharmacology·Clive Page, Simon Pitchford
Dec 18, 2013·Circulation·Sam SchulmanUNKNOWN RE-COVER II Trial Investigators
Jan 5, 2014·Cardiovascular Research·Henri M H SpronkHugo Ten Cate
Jul 12, 2014·Arteriosclerosis, Thrombosis, and Vascular Biology·Travis J GouldPatricia C Liaw
Dec 3, 2014·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Eniko BerkesMona Saffarzadeh
Nov 11, 2015·Heart·Philip JosephUNKNOWN RE-LY Investigators
Nov 15, 2015·Heart·Hugo Ten Cate
Jan 30, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Norbert GerdesEsther Lutgens
Mar 10, 2016·Expert Opinion on Drug Safety·Daniel CaldeiraJoão Costa
Dec 9, 2016·Haematologica·Susanne PfeilerBernd Engelmann
Jan 20, 2018·Circulation Research·Milka KoupenovaJane E Freedman

❮ Previous
Next ❯

Methods Mentioned

BETA
PMA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of the American College of Cardiology
Magda Heras
The American Journal of Cardiology
John R Kapoor
Medizinische Monatsschrift für Pharmazeuten
Heike Oberpichler-Schwenk
© 2022 Meta ULC. All rights reserved