The effects of DDT, DDOH-palmitic acid, and a chlorinated paraffin on muscarinic receptors and the sodium-dependent choline uptake in the central nervous system of immature mice

Toxicology and Applied Pharmacology
P Eriksson, A Nordberg

Abstract

Ten-day-old NMRI mice were given a single peroral dose of 1.4 mumol/kg body wt of either of the substances of DDT (0.5 mg), DDOH-PA (a DDT metabolite conjugated to palmitic acid; 0.7 mg), or polychlorohexadecane (PCHD, a synthesized chlorinated paraffin; 1.0 mg). The mice were killed either 24 hr or 7 days after treatment, and crude synaptosomal fractions (P2) were prepared from the cerebral cortex and hippocampus. The density of the muscarinic receptors was assayed by measuring quinuclidinyl benzilate ([3H]QNB) specifically bound in the P2 fraction. A significant increase in the specific [3H]QNB binding was observed in the cerebral cortex in DDT- and DDOH-PA-treated mice 7 days after treatment, compared to control. These results were further explored by determining the ratio of high- and low-affinity binding sites by using an agonist (carbachol)-antagonist ([3H]QNB) competition assay. A significant decrease in the percentage of high-affinity binding sites and a corresponding increase in the percentage of low-affinity binding sites, compared to control, were observed after DDT and DDOH-PA treatment. The presynaptically sodium-dependent choline uptake system also was studied. In mice receiving PCHD there was a significant decrea...Continue Reading

Citations

Nov 19, 1991·Brain Research. Developmental Brain Research·A NordbergP Eriksson
Jan 1, 1991·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·P Eriksson
May 15, 1996·Environmental Toxicology and Pharmacology·P Eriksson, A Fredriksson
Oct 1, 1990·Journal of Toxicology and Environmental Health·H BouwmanP J Becker
Sep 15, 2009·Environmental Health Perspectives·Brenda EskenaziDarwin Stapleton
Sep 29, 2015·Environment International·Fraser W GasparBrenda Eskenazi
Nov 23, 2006·Scandinavian Journal of Caring Sciences·Ingrid Snellman, Karin Wikblad
Sep 3, 2020·EFSA Journal·UNKNOWN EFSA Panel on Contaminants in the Food Chain (CONTAM)Elsa Nielsen
Dec 16, 1997·Journal of Toxicology and Environmental Health·H J ClewellJ M Gearhart
May 12, 2006·Critical Reviews in Toxicology·E Mariussen, F Fonnum
Sep 2, 2021·The Science of the Total Environment·Lei DingYahui Li

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