PMID: 18724006Aug 30, 2008Paper

The effects of dexmedetomidine on human neutrophil apoptosis

Biomedical Research
Hiroaki KishikawaAtsuhiro Sakamoto

Abstract

The purpose of the present study was to evaluate the effect of dexmedetomidine, used as a sedative in the intensive care unit, on human neutrophil apoptosis and superoxide production in vitro. Neutrophils from healthy volunteers were incubated in different concentrations of dexmedetomidine (1, 10 and 100 ng/mL). Apoptosis was assessed by Hoechst 33342 staining, caspase activities and loss of mitochondrial transmembrane potential (MTP). Superoxide production was determined by the WST-1 assay. After 24 h of incubation, dexmedetomidine accelerated neutrophil apoptosis in a dose-dependent manner and 100 microM yohimbine did not inhibit the apoptosis. Treatment with 100 ng/mL of dexmedetomidine significantly enhanced the activation of caspases-3/7, -8 and -9, and also markedly increased the number of neutrophils with decreased MTP. At 24 h, the suppression of superoxide production was dependent on dexmedetomidine concentrations. However, a clinically relevant concentration (1 ng/mL) of dexmedetomidine did not affect neutrophil apoptosis and superoxide production. These results suggest that high doses of dexmedetomidine induce apoptosis without alpha(2)-adrenoceptors stimulus and inhibit superoxide production after long-term incubati...Continue Reading

References

Jul 15, 1983·Biochemical Pharmacology·J O Panosian, G V Marinetti
Jul 1, 1993·The Journal of Clinical Investigation·M K WhyteC Haslett
Feb 11, 1999·Anesthesia and Analgesia·K NishinaY Niwa
Jun 29, 2000·Annual Review of Biochemistry·W C EarnshawS H Kaufmann
Jan 17, 2003·American Journal of Physiology. Cell Physiology·Tania C Pithon-CuriRui Curi
Feb 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gianluca FossatiSteven W Edwards
Oct 21, 2004·Biochemical Society Transactions·D Scheel-ToellnerJ M Lord
Jul 11, 2006·Lancet·K A BrownD F Treacher

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Citations

Mar 10, 2011·Anesthesia and Analgesia·Koichi YukiMotomu Shimaoka
Jun 13, 2009·Acta Anaesthesiologica Scandinavica·M CanS Acikgoz
Mar 6, 2017·Veterinary Anaesthesia and Analgesia·Francesco ManciniElisabetta Chiaradia
Sep 27, 2013·Hepatitis Monthly·Ali Dabbagh, Samira Rajaei
Aug 17, 2016·Anesthesia and Analgesia·Jia-Ren LiuSulpicio G Soriano
Aug 13, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ada María Herrera-GarcíaFederico Díaz-González
Mar 10, 2020·Anesthesia and Analgesia·Yusuke MitsuiKoichi Yuki

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