The effects of diaspirin-crosslinked hemoglobin on the assessment of immunohematology profiles

Transfusion
A J ReppucciD L Balma

Abstract

Extensive studies have been conducted on the in vitro effects of diaspirin-crosslinked hemoglobin (DCLHb) in biochemical, hematologic, hemostatic, and blood banking (immunohematologic) methods. The absence of red cell antigens or plasma and/or serum antibodies allows DCLHb to be used as "universal-donor" material. This study evaluates the effects of DCLHb on the accurate assessment of the immunohematologic profile (ABO and Rh blood grouping, antibody screen, and crossmatching). DCLHb, 7.4 g per dL in an electrolyte solution, was mixed in vitro with human whole blood, representing the blood types A Rh-positive. A Rh-negative, B Rh-positive, B Rh-negative, O Rh-positive, O Rh-negative, and AB Rh-positive. Two concentrations of DCLHb were tested: 10-percent (0.74 g/dL) and 30-percent (2.22 g/dL). Controls were prepared by adding a 5-percent albumin solution to aliquots of whole blood in volumes equivalent to those used in preparing the DCLHb dilutions. Serum and/or red cell suspensions from these admixed samples were analyzed for their ABO and Rh blood groups, the presence of unexpected antibodies (antibody screen), and compatibility in crossmatch testing. DCLHb added to whole blood in vitro had no effect on the accurate interpret...Continue Reading

Citations

Mar 6, 2003·Vox Sanguinis·Donat R Spahn
Dec 19, 2019·Frontiers in Bioengineering and Biotechnology·Nan ZhangQiang Ma
Jun 1, 2005·Anesthesiology Clinics of North America·Caroline Thyes, Donat R Spahn
May 23, 2008·Best Practice & Research. Clinical Anaesthesiology·Roman Kocian, Donat R Spahn

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