The effects of dietary macronutrient composition on lipid metabolism-associated factor gene expression in the adipose tissue of chickens are influenced by fasting and refeeding

BMC Obesity
Guoqing WangElizabeth R Gilbert

Abstract

Broiler chickens are compulsive feeders that become obese as juveniles and are thus a unique model for metabolic disorders in humans. However, little is known about the relationship between dietary composition, fasting and refeeding and adipose tissue physiology in chicks. Our objective was to determine how dietary macronutrient composition and fasting and refeeding affect chick adipose physiology during the early post-hatch period. Chicks were fed one of three isocaloric diets after hatch: high-carbohydrate (HC; control), high-fat (HF; 30% of ME from soybean oil) or high-protein (HP; 25% vs. 22% crude protein). At 4 days post-hatch, chicks were fed (continuous ad libitum access to food), fasted (3 h food withdrawal), or refed (fasted for 3 h and refed for 1 h). Subcutaneous, clavicular, and abdominal adipose tissue was collected for histological analysis and to measure gene expression, and plasma to measure non-esterified fatty acid (NEFA) concentrations (n = 6-10 per group). Adipose tissue weights were reduced in chicks that were fed the HP diet and adipocyte diameter was greater in the adipose tissue of chicks that ate the HF diet. Consumption of diets differing in protein and fat content also affected gene expression; mRNAs...Continue Reading

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Citations

Jun 20, 2017·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Guoqing WangElizabeth R Gilbert
Mar 7, 2018·General and Comparative Endocrinology·Guoqing WangElizabeth R Gilbert
Mar 17, 2020·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Yang XiaoElizabeth R Gilbert

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Primer Express
NIS
Elements Advanced Research
JMP Pro11
JMP

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