The effects of different levels of catalase and superoxide dismutase in modified Beltsville extender on rooster post-thawed sperm quality

Cryobiology
Mahmood Reza AminiMohammad Mehdi Nabi

Abstract

Oxidative damage of sperm by means of reactive oxygen species generated by the cellular components of semen is one of the main reasons for decreased sperm motility and fertility during the freeze-thawing process. This study was conducted to determine the influence of catalase (CAT) and superoxide dismutase (SOD) on rooster sperm motility, viability and MDA level after freezing and thawing. Semen samples from 10 sexually-mature Ross 308 breeder roosters were collected and pooled, divided into nine equal parts and diluted with modified Beltsville extender containing no antioxidants (control), or supplemented with 50, 100, 200 and 300 μg/mL CAT, or 50, 100, 200 and 300 U/mL SOD. After thawing, sperm motility and motion parameters were assessed using a CASA system. Sperm viability and MDA level were assessed by eosin-nigrosin and MDA test, respectively. The results of this experiment showed that the extender supplemented with 100 and 200 μg CAT, and 50 U SOD had the highest sperm motility (P<0.05) in sperm motility. Also, addition 100, 200 and 300 μg CAT, and 50 U SOD can improve significantly viability after freeze-thaw. Extender supplemented with 100 μg CAT had significantly lower MDA level compared to control and 300 μg CAT. In ...Continue Reading

References

May 1, 1993·Journal of Reproduction and Fertility·R J AitkenD Buckingham
Jan 1, 1996·Reproduction, Fertility, and Development·W M Maxwell, T Stojanov
Nov 22, 1997·Biology of Reproduction·S CeroliniL G Cavalchini
Sep 23, 2003·Molecular Reproduction and Development·Christelle BréqueJean-Pierre Brillard
Apr 14, 2005·Theriogenology·Elizabeth BreiningerMartha T Beconi
Jun 7, 2005·American Journal of Veterinary Research·Julie BaumberJennifer J Linfor
Oct 7, 2010·Biological Trace Element Research·Urszula Marzec-WróblewskaJacek Michałkiewicz
Jun 19, 2013·Cryobiology·Agnieszka PartykaMałgorzata Ochota

❮ Previous
Next ❯

Citations

Sep 24, 2016·Animal Reproduction Science·Soroush SafaHossein Janmohammadi
Sep 27, 2018·Oxidative Medicine and Cellular Longevity·Michał DobrakowskiAleksandra Kasperczyk
Jul 12, 2017·Reproduction in Domestic Animals = Zuchthygiene·M ZhandiM Sharafi
Jan 11, 2020·Free Radical Biology & Medicine·Kaushiki KadamBini Ramachandran
Feb 20, 2021·Research in Veterinary Science·Ana Patricia Alves LeãoMarcio Gilberto Zangeronimo
Oct 2, 2019·Theriogenology·Masoumeh SalehiAbdolhossein Shahverdi
Aug 8, 2021·Journal of Animal Physiology and Animal Nutrition·Sara F FoudaIbrahim T El-Ratel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.