The effects of different levels of vitamin E and vitamin C in modified Beltsville extender on rooster post-thawed sperm quality

Cell and Tissue Banking
Mahmood Reza AminiMohammad Mehdi Nabi

Abstract

Oxidative damage of sperm by means of reactive oxygen species generated by the cellular components of semen is one of the main reason of declined motility and fertility of sperm during the freeze-thawing process. This study was conducted to determine the influence of vitamin C and vitamin E on rooster post-thawed sperm motility, viability and malondialdehyde (MDA) level. Semen samples from 10 sexually-mature Ross 308 breeder roosters were collected and pooled, divided into nine equal parts and diluted with modified Beltsville extender containing with no antioxidants (control), or containing 100 (C100), 200 (C200), 400 (C400), 800 (C800) µg/mL vitamin C, and 2 (E2), 5 (E5), 10 (E10) and 15 (E15) µg/mL vitamin E. After thawing, total and progressive sperm motility, sperm viability and semen MDA level were assessed. The results shown that C200 and E5 extenders resulted in higher total motility (p < 0.05) compared to other extenders, with exception of E10 extender. Progressive motility was higher in E5 extender (p < 0.05) compared to other extenders, with exception of C200 and E10 extenders. Also, C200 and E5 extenders resulted in higher viability of post-thawed spermatozoa (p < 0.05) compared to other extenders. Finally, the resul...Continue Reading

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Sep 24, 2016·Animal Reproduction Science·Soroush SafaHossein Janmohammadi
Mar 29, 2020·Animal Science Journal = Nihon Chikusan Gakkaihō·Dejun XuQingwang Li
Jul 12, 2017·Reproduction in Domestic Animals = Zuchthygiene·M ZhandiM Sharafi
Feb 20, 2021·Research in Veterinary Science·Ana Patricia Alves LeãoMarcio Gilberto Zangeronimo
Jul 6, 2021·Veterinary World·Ghadeer Sabah Bustani, Falah Hasan Baiee

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