The effects of dynamic compressive loading on human mesenchymal stem cell osteogenesis in the stiff layer of a bilayer hydrogel

Journal of Tissue Engineering and Regenerative Medicine
Aaron H AzizStephanie J Bryant

Abstract

Bilayer hydrogels with a soft cartilage-like layer and a stiff bone-like layer embedded with human mesenchymal stem cells (hMSCs) are promising for osteochondral tissue engineering. The goals of this work were to evaluate the effects of dynamic compressive loading (2.5% applied strain, 1 Hz) on osteogenesis in the stiff layer and spatially map local mechanical responses (strain, stress, hydrostatic pressure, and fluid velocity). A bilayer hydrogel was fabricated from soft (24 kPa) and stiff (124 kPa) poly (ethylene glycol) hydrogels. With hMSCs embedded in the stiff layer, osteogenesis was delayed under loading evident by lower OSX and OPN expressions, alkaline phosphatase activity, and collagen content. At Day 28, mineral deposits were present throughout the stiff layer without loading but localized centrally and near the interface under loading. Local strains mapped by particle tracking showed substantial equivalent strain (~1.5%) transferring to the stiff layer. When hMSCs were cultured in stiff single-layer hydrogels subjected to similar strains, mineralization was inhibited. Finite element analysis revealed that hydrostatic pressures ≥~600 Pa correlated to regions lacking mineralization in both hydrogels. Fluid velocities ...Continue Reading

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Citations

Aug 1, 2019·Biomaterials Science·Anastasiia KashirinaJinsong Leng
Oct 20, 2020·Advanced Healthcare Materials·Rachel L WilmothStephanie J Bryant
Feb 16, 2021·Frontiers in Bioengineering and Biotechnology·Sarah DavisTosca Roncada
Jan 19, 2021·ACS Biomaterials Science & Engineering·Zhinan MaoSujun Wu

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