The Effects of EDTA on Blood Clot in Regenerative Endodontic Procedures

Journal of Endodontics
Peerapohn TaweewattanapaisanKajohnkiart Janebodin

Abstract

In regenerative endodontic procedures (REPs), a blood clot acts as a natural scaffold for regenerating dental pulp tissue. In current protocols, 17% EDTA is recommended for liberating growth factors from root dentin. Although EDTA affects clot formation in periodontal studies, the anticoagulant effect of EDTA has not been shown in REPs. Therefore, this study aimed to evaluate the effects of 17% EDTA on the characteristics and fiber density of blood clots using in vitro dentin blocks. The roots of 35 human mandibular premolars were prepared to simulate open apices and irrigated with the following protocols: (1) normal saline solution (NSS), (2) EDTA (1 minute) + NSS (E1N), (3) EDTA (5 minutes) + NSS (E5N), (4) EDTA (1 minute) (E1), and (5) EDTA (5 minutes) (E5). The roots were split, and human blood was placed. The characteristics and fibrin density of clots were observed using a scanning electron microscope. Fibrin densities in all irrigation groups were evaluated using ImageJ software (National Institutes of Health, Bethesda, MD) and statistically analyzed using the Friedman test and the Kruskal-Wallis test with Bonferroni adjustment. Samples in the NSS, E1N, and E5N groups revealed denser fibers with an abundance of erythrocy...Continue Reading

References

Nov 5, 1999·Biophysical Journal·E A RyanL Lorand
Apr 16, 2004·Journal of Endodontics·Francisco Banchs, Martin Trope
May 19, 2007·Journal of Endodontics·Blayne ThibodeauMartin Trope
Dec 25, 2009·Journal of Oral Science·Shehab El-Din M Saber
Jun 1, 2013·Journal of Clinical Pathology·Jose Manuel VagaceGuillermo Gervasini
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Apr 27, 2016·Clinical Oral Investigations·M WidbillerK M Galler
Oct 30, 2016·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Omid DianatNazanin Bahrololumi

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