The effects of electrospun substrate-mediated cell colony morphology on the self-renewal of human induced pluripotent stem cells

Biomaterials
Maricela MaldonadoJin Nam

Abstract

The development of xeno-free, chemically defined stem cell culture systems has been a primary focus in the field of regenerative medicine to enhance the clinical application of pluripotent stem cells (PSCs). In this regard, various electrospun substrates with diverse physiochemical properties were synthesized utilizing various polymer precursors and surface treatments. Human induced pluripotent stem cells (IPSCs) cultured on these substrates were characterized by their gene and protein expression to determine the effects of the substrate physiochemical properties on the cells' self-renewal, i.e., proliferation and the maintenance of pluripotency. The results showed that surface chemistry significantly affected cell colony formation via governing the colony edge propagation. More importantly, when surface chemistry of the substrates was uniformly controlled by collagen conjugation, the stiffness of substrate was inversely related to the sphericity, a degree of three dimensionality in colony morphology. The differences in sphericity subsequently affected spontaneous differentiation of IPSCs during a long-term culture, implicating that the colony morphology is a deciding factor in the lineage commitment of PSCs. Overall, we show t...Continue Reading

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Citations

Jan 18, 2016·Journal of the Mechanical Behavior of Biomedical Materials·Christopher B HornerJin Nam
Feb 10, 2016·Biomicrofluidics·Yihe WangEugenia Kumacheva
Jun 4, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Mattias LeinoVéronique Chotteau
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Nov 25, 2020·Tissue Engineering and Regenerative Medicine·Jasmeet Kaur Virdi, Prasad Pethe
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Jul 6, 2021·Biomaterials Science·Fábio F F GarrudoFrederico Castelo Ferreira
Nov 13, 2019·ACS Applied Materials & Interfaces·Christopher B HornerJin Nam

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