PMID: 6350599Jul 1, 1983Paper

The effects of embedding in water-soluble plastics on the final dimensions of liver sections

Journal of Microscopy
J G Hanstede, P O Gerrits

Abstract

This paper describes the morphometric changes liver samples undergo in the course of fixation, dehydration, infiltration and embedding in different mixtures of water-soluble plastics. The plastics used were: three different mixtures of glycolmethacrylate (GMA) and the commercially available material JB4. Buffered formaldehyde fixation did not produce significant morphometric changes in the liver specimens. Dehydration obviously affects the volume of the liver specimen (linear shrinkage about 9.3%). The dehydration is followed by an infiltration phase. During this phase a slight swelling (linear, 2-5%) occurs. The final polymerization of the plastic resulted in a further linear shrinkage of 1-2%. The influence of different technical factors on the stretching of sections of pure plastic and of plastic embedded liver appeared to be considerable. The difference in stretching between 2 and 3 micron sections has been studied. A significant influence of temperature upon section stretching was noted. Sections of all plastic mixtures stretched at a temperature of 293 K showed 3% more linear stretch than at a temperature of 333 K. Differences between the four plastic mixtures are discussed. Correction factors must be used in morphometric...Continue Reading

References

Citations

Jan 1, 1991·Progress in Neurobiology·J P Royet
Feb 24, 1999·Journal of Anatomy·W Meyer, G Baumgärtner
Apr 11, 1997·Journal of Toxicology and Environmental Health·M G MénacheF J Miller
Jul 1, 1993·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·G UhrM Schwenk
Jun 1, 1986·The American Journal of Anatomy·W MeyerC Schlesinger
Feb 13, 2001·Kidney International·J F Bertram
Jun 1, 1994·Kidney International·J M BasgenS M Mauer
Feb 9, 2016·Interface Focus·Monica E SusiloJeffrey W Ruberti
Jan 16, 2009·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Christian MühlfeldT M Mayhew
Sep 16, 2015·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Stine HasselholtJytte Overgaard Larsen
Feb 7, 2009·Journal of Microscopy·W MeyerHassan Y Naim
Jul 10, 2007·Anatomia, histologia, embryologia·M Ferreira-MedeirosE M Correa-Gillieron
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Dec 1, 1995·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M Ladekarl, H Svanholm
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Jan 28, 2017·Journal of Applied Physiology·Julia SchipkeChristian Mühlfeld
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Oct 9, 2018·Microscopy Research and Technique·Tomasz H Skrzypek, Waldemar Kazimierczak
May 8, 1999·Journal of the American Society of Nephrology : JASN·J R Nyengaard

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