The effects of enamel matrix derivative on the proliferation and differentiation of human mesenchymal stem cells

Clinical Oral Implants Research
Seong-Suk JueBaeksoo Lee

Abstract

This study was designed to investigate the effect of enamel derivative matrix (EMD) on the proliferation, mineralization, and differentiation of human mesenchymal stem cells (hMSCs). For the proliferation assay, water-soluble tetrazolium salt-8 tests were carried out after culturing for 24 and 48 h. For the evaluation of mineralization, Alizarin red S (ARS) tests were performed after 21 days of culturing in an osteogenic medium. In order to investigate some of the bone-related proteins, namely type I collagen (Col I A2), bone sialoprotein (BSP), and bone gamma-carboxyglutamate (Gla) protein (BGLAP, osteocalcin), real-time polymerase chain reaction (RT-PCR) tests were carried out after 2, 3, and 4 weeks of culturing, respectively. The activity of proliferation and mineralization increased significantly depending on the concentration of EMD (P<0.05). In the control group, the expression of Col I A2 decreased, but EMD enhanced its expression over time and was correlated to the concentration. The amount of expression of BSP in this group increased over time, but EMD strikingly suppressed its expression in the fourth week. As well, the amount of expression of BGLAP increased as the culture duration lengthened in the control group. H...Continue Reading

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Citations

Sep 29, 2011·Journal of Periodontology·Kotaro TanimotoKazuo Tanne
Oct 2, 2013·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Karim M Fawzy El-SayedSebastian Paris
Nov 29, 2013·Journal of Periodontology·Mônica G CorrêaEnilson A Sallum
Sep 18, 2014·Frontiers in Bioengineering and Biotechnology·Lisanne C GroeneveldtEric Farrell
Apr 11, 2017·Journal of Periodontal Research·S A TassiC C Villar
May 12, 2018·International Journal of Oral Science·Sema S HakkiWerner Götz
Jan 21, 2021·Biomedical Materials·Yifeng BianYifei Du
Jun 3, 2021·International Journal of Molecular Sciences·Benedikt EggersMarjan Nokhbehsaim

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