The Effects of Endoplasmic-Reticulum-Resident Selenoproteins in a Nonalcoholic Fatty Liver Disease Pig Model Induced by a High-Fat Diet

Nutrients
Pengzu WangAnshan Shan

Abstract

The present study aimed to investigate the intervention of selenium in the oxidative stress and apoptosis of pig livers, which were induced by a high-fat diet, and the effects of four endoplasmic reticulum (ER)-resident selenoproteins in the process. A 2×4 design trial was conducted that included two dietary fat levels (BD = basal diet and HFD = high-fat diet) and four dietary Se supplementation levels (0, 0.3, 1.0, and 3.0 mg/kg of the diet, in the form of sodium selenite (Na2SeO3)). Our results indicated that the HFD significantly increased the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum, as well as the degree of steatosis, the content of malondialdehyde (MDA), the apoptotic rate, and the level of mRNA caspase-3 in the liver compared to their BD counterparts (p < 0.05). Moreover, these parameters in the HFD groups were more significantly reduced (p < 0.05) for a Se concentration of 1.0 mg/kg than for the other concentrations. Further, for both the BD and HFD, the groups supplemented with 1.0 mg/kg Se showed the highest mRNA level of selenoprotein S. In conclusion, the consumption of an HFD can induce oxidative damage and apoptosis in the liver. This shows that the supplementa...Continue Reading

References

Jan 7, 1998·The Journal of Cell Biology·K G WolterR J Youle
Apr 18, 1998·Gastroenterology·C P Day, O F James
Apr 14, 1999·Cell Death and Differentiation·A G Porter, R U Jänicke
May 29, 1999·Gastroenterology·C A MatteoniA J McCullough
Jun 23, 2000·Annals of the New York Academy of Sciences·T C Stadtman
Oct 5, 2001·The Journal of Allergy and Clinical Immunology·K C Zimmermann, D R Green
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 7, 2002·Aquatic Toxicology·Julian E Spallholz, David J Hoffman
May 29, 2002·Diabetes·Ken WalderGreg R Collier
Jul 23, 2002·The Proceedings of the Nutrition Society·Margaret P Rayman, Margaret P Rayman
Jan 14, 2003·Annual Review of Nutrition·Donna M Driscoll, Paul R Copeland
Apr 18, 2003·Human Molecular Genetics·Nathalie PetitPascale Guicheney
May 31, 2003·Science·Gregory V KryukovVadim N Gladyshev
Jul 1, 1957·American Journal of Clinical Pathology·S REITMAN, S FRANKEL
Jan 31, 2004·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Nadya MorozovaMarla J Berry
Feb 27, 2004·The American Journal of Clinical Nutrition·Charles S LieberLeonore M DeCarli
Feb 27, 2004·The Journal of Biological Chemistry·Naoya YahagiNobuhiro Yamada
Aug 20, 2005·Diabetic Medicine : a Journal of the British Diabetic Association·L A Adams, P Angulo
Apr 15, 2006·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Raymond F BurkDaniel W Byrne
Sep 14, 2006·The Journal of Clinical Endocrinology and Metabolism·Kristina M Utzschneider, Steven E Kahn
Oct 13, 2006·World Journal of Gastroenterology : WJG·Anatol PanasiukDanuta Prokopowicz
Jan 11, 2007·Biochemical and Biophysical Research Communications·Kee-Hong KimAhmed H Kissebah
Jun 5, 2007·Clinics in Liver Disease·Janus P Ong, Zobair M Younossi
Feb 1, 1997·Trends in Cell Biology·H L Pahl, P A Baeuerle
Oct 20, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·January N BaumgardnerMartin J J Ronis
Jan 17, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Luca GrumolatoYoussef Anouar
Mar 18, 2008·The Veterinary Journal·F J Mulligan, M L Doherty
Apr 15, 2008·Experimental Biology and Medicine·Jannine K PattersonDennis D Miller
Dec 31, 2008·Mutation Research·Rodrigo FrancoMihalis I Panayiotidis
Feb 20, 2009·Journal of Gastroenterology and Hepatology·Geoffrey C FarrellLisa M Sedger
Mar 4, 2009·The Journal of Biological Chemistry·Diane E HandyJoseph Loscalzo
Apr 14, 2009·Molecular and Cellular Biochemistry·Pavitra Ranawat, M P Bansal
Aug 10, 2010·Archives of Biochemistry and Biophysics·Shaoqing DuKaixun Huang

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Methods Mentioned

BETA
light microscopy
electrophoresis
PCR
MDA

Software Mentioned

Primer Premier
Image
Pro Plus
BLAST
SPSS

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