PMID: 6162861Apr 1, 1981Paper

The effects of fasting and feeding on preproinsulin messenger RNA in rats

The Journal of Clinical Investigation
S J GiddingsM A Permutt

Abstract

The purpose of these experiments was to determine whether alterations in preproinsulin messenger (m)RNA activity could account for changes in insulin biosynthesis during fasting and refeeding. Rats were fasted 4 d and then fed for 6, 8, 24, or 48 h. With fasting, body weight decreased 25%, plasma glucose decreased from 6.1 to 2.2 mM, and pancreatic insulin content fell to 40% that of fed animals. Islet RNA decreased to 50% and protein to 55% that of control animals, while islet DNA content remained unchanged. After 6 h of refeeding, islet RNA content increased and was not significantly different from controls. Total islet and preproinsulin mRNA activity was estimated with an mRNA-dependent wheat germ cell-free protein synthesizing system. Preproinsulin and total protein synthesis was linearly dependent upon added RNA at concentrations up to 3 mug. Preproinsulin was identified by its mobility on SDS polyacrylamide gel electrophoresis and by hybrid arrested translation of preproinsulin mRNA. After an 18-h fast, islet mRNA activity decreased 33%; after 4 d mRNA activity decreased to 66% below that of control fed animals. Preproinsulin mRNA activity was decreased, but to a lesser extent, accounting for 20% of total islet protein in...Continue Reading

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Citations

Aug 1, 1992·Molecular and Cellular Endocrinology·B M WentworthL Villa-Komaroff
Oct 1, 1981·Clinical Biochemistry·A PermuttP Rotwein
May 3, 1984·The New England Journal of Medicine·D C RobbinsA H Rubenstein
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K KakitaM A Permutt
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·F C SchuitD G Pipeleers
Dec 12, 2012·The Journal of Biological Chemistry·Leena HaatajaPeter Arvan
Dec 1, 1991·The Proceedings of the Nutrition Society·K DochertyS W Knight
Aug 15, 2000·Annual Review of Nutrition·I R Sanderson, S Naik
Feb 1, 1992·The Journal of Clinical Investigation·L KoranyiM A Permutt
Dec 13, 2007·PloS One·Emilie StolarczykArmelle Leturque
Jun 27, 2013·Diabetes·Christopher J RhodesSteven E Kahn
Aug 15, 1991·Biochemical and Biophysical Research Communications·F C SchuitD G Pipeleers
Dec 11, 2003·American Journal of Physiology. Endocrinology and Metabolism·M A MartínC Alvarez
Dec 16, 2017·Endocrinology·Brandon B BolandChristopher J Rhodes
Aug 1, 1993·The American Journal of Physiology·S J GiddingsR A Shalwitz
Jun 30, 1982·Biochemical and Biophysical Research Communications·J Brunstedt, S J Chan
Dec 30, 1986·Biochemical and Biophysical Research Communications·H FukumotoH Imura

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